Identification of differentially expressed proteins in the heart of translationally controlled tumor protein over-expressing transgenic mice

Myeong Sook Cheon, Jae kyung Suh, Min Jeong Kim, Seong Hwan Kim, Kyunglim Lee

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

We previously reported that transgenic (TG) mice over-expressing translationally controlled tumor protein (TCTP) developed systemic arterial hypertension at about 6 weeks after birth. In the present study, we identi?ed, using proteomics technologies, 24 other proteins that were differentially expressed in the heart of TCTP over-expressing TG mice. These 24 proteins are involved in a variety of biological processes such as reactive oxygen species metabolism, cytoskeleton organization, fatty acid metabolism, amino acid metabolism and energy metabolism. We determined protein expression levels of the peroxiredoxin (Prx)2, Prx3, myosin light chain 1, stress protein (heat shock protein) 25K, and T-complex protein 1 alpha subunit by western blot analysis. Over-expression of TCTP probably regulates the expression of other proteins which play a pivotal role in a variety of cellular functions in TCTP over-expressing TG mice.

Original languageEnglish
Pages (from-to)1091-1099
Number of pages9
JournalBiomedical Chromatography
Volume22
Issue number10
DOIs
StatePublished - 2008

Keywords

  • 2-DE
  • Heart
  • MALDI-TOF MS
  • Transgenic mice
  • Translationally controlled tumor protein

Fingerprint

Dive into the research topics of 'Identification of differentially expressed proteins in the heart of translationally controlled tumor protein over-expressing transgenic mice'. Together they form a unique fingerprint.

Cite this