Identification of cytoprotective constituents of the flower buds of Tussilago farfara against glucose oxidase-induced oxidative stress in mouse fibroblast NIH3T3 cells and human keratinocyte HaCaT cells

Unwoo Kang, Jiyoung Park, Ah Reum Han, Mi Hee Woo, Je Hyun Lee, Sang Kook Lee, Tong Shin Chang, Hyun Ae Woo, Eun Kyoung Seo

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

A new cytoprotective compound, 1-[(4S)-3,4-dihydro-4-hydroxy-2,2-dimethyl-2H-1-benzopyran-6-yl]-ethanone (1) was isolated from the flower buds of Tussilago farfara L. (Compositae), together with eight known compounds, 3,4-dicaffeoyl isoquinic acid (2), trans-cinnamic acid (3), 4-hydroxyacetophenone (4), 4,5-dicaffeoylquinic acid methyl ester (5), 3,5-dicaffeoylquinic acid methyl ester (6), 4-hydroxybenzoic acid (7), isoquercetrin (8), and ligucyperonol (9). Compounds 2-4 were found in this plant for the first time. The isolates 1-9, were tested for their cytoprotective activities against glucose oxidase-induced oxidative stress in mouse fibroblast NIH3T3 cells and human keratinocyte HaCaT cells. Among them, 1 and 3 showed significant cytoprotective activities as determined by MTT assay and lactate dehydrogenase leakage, indicating their possibility as the potent cytoprotective agents. The structure of 1 was determined by spectroscopic data analysis including 1D- and 2D-NMR experiments, and its absolute configuration was elucidated by a circular dichroism.

Original languageEnglish
Pages (from-to)474-480
Number of pages7
JournalArchives of Pharmacal Research
Volume39
Issue number4
DOIs
StatePublished - 1 Apr 2016

Bibliographical note

Funding Information:
This research was funded by a Grant (12172MFDS989) from Ministry of Food and Drug Safety in 2014, and was also supported by Global PhD Fellowship Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2012H1A2A1017710).

Publisher Copyright:
© 2016 The Pharmaceutical Society of Korea.

Keywords

  • Compositae
  • Cytoprotection
  • Oxidative stress
  • Phenolic compound
  • Tussilago farfara

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