Identification of Antiangiogenic Potential and Cellular Mechanisms of Napyradiomycin A1 Isolated from the Marine-Derived Streptomyces sp. YP127

Ji Sun Hwang, Geum Jin Kim, Hyun Gyu Choi, Min Cheol Kim, Dongyup Hahn, Joo Won Nam, Sang Jip Nam, Hak Choel Kwon, Jungwook Chin, Sung Jin Cho, Hayoung Hwang, Hyukjae Choi

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Angiogenesis is the process of new blood vessel formation. Excessive angiogenesis is a critical factor in the progression of cancer, macular degeneration, and other chronic inflammatory diseases. When investigating the effects of crude extracts of cultured marine microorganisms, an extract of the cultured Streptomyces sp. YP127 strain was found to inhibit human umbilical vein endothelial cell (HUVEC) tube formation. Bioassay-guided fractionation and spectroscopic data analyses led to the identification of napyradiomycin A1 (1) as an antiangiogenic component of the extract. Compound 1 inhibited HUVEC tube formation in a concentration-dependent manner. It inhibited endothelial cell proliferation but did not affect human dermal fibroblast proliferation. Compound 1 also suppressed migration and invasion of vascular endothelial cells. In addition, compound 1 suppressed vascular endothelial cadherin expression and increased the permeability of the endothelial cell membrane. These results suggested that compound 1 modulates cell permeability and inhibits the angiogenesis of endothelial cells.

Original languageEnglish
Pages (from-to)2269-2275
Number of pages7
JournalJournal of Natural Products
Volume80
Issue number8
DOIs
StatePublished - 25 Aug 2017

Bibliographical note

Funding Information:
This research was supported by the Bio & Medical Technology Development Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (Nos. 2014M3A9D9033717 and 2014R1A1A2057302).

Publisher Copyright:
© 2017 The American Chemical Society and American Society of Pharmacognosy.

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