Identification of a novel ionizing radiation-induced nuclease, AEN, and its functional characterization in apoptosis

Ji Hyun Lee; Yeon A. Koh; Chul Koo Cho; Su Jae Lee; Yun Sil Lee; Sangwoo Bae

doi:10.1016/j.bbrc.2005.08.264

Identification of a novel ionizing radiation-induced nuclease, AEN, and its functional characterization in apoptosis

Ji Hyun Lee, Yeon A. Koh, Chul Koo Cho, Su Jae Lee, Yun Sil Lee, Sangwoo Bae

Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

To investigate ionizing radiation response, we screened genes that exhibit higher expression following γ irradiation. We report here the isolation and functional characterization of a novel ionizing radiation-induced gene, AEN. Sequence analysis of AEN revealed exonuclease domain highly similar to that of exonuclease III. The AEN protein revealed DNase activity by cleaving various DNA substrates. Subcellular distribution of AEN exhibited nuclear colocalization with apoptotic nucleases such as CAD and AIF following irradiation. Moreover AEN distribution revealed perinuclear staining pattern which could be seen with other apoptotic nucleases. Irradiation of AEN-expressing cells resulted in synergistic increase of apoptosis whereas AEN deletion mutant in exonuclease domain did not. Our data, thus, suggest that radiation-induced AEN cleaves DNA in concert with other apoptotic nucleases and thereby enhances apoptosis following ionizing irradiation.

Original language	English
Pages (from-to)	39-47
Number of pages	9
Journal	Biochemical and Biophysical Research Communications
Volume	337
Issue number	1
DOIs	https://doi.org/10.1016/j.bbrc.2005.08.264
State	Published - 11 Nov 2005

Keywords

Apoptosis
Apoptosis enhancing nuclease
DNase
Ionizing radiation
Radiation response

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title = "Identification of a novel ionizing radiation-induced nuclease, AEN, and its functional characterization in apoptosis",

abstract = "To investigate ionizing radiation response, we screened genes that exhibit higher expression following γ irradiation. We report here the isolation and functional characterization of a novel ionizing radiation-induced gene, AEN. Sequence analysis of AEN revealed exonuclease domain highly similar to that of exonuclease III. The AEN protein revealed DNase activity by cleaving various DNA substrates. Subcellular distribution of AEN exhibited nuclear colocalization with apoptotic nucleases such as CAD and AIF following irradiation. Moreover AEN distribution revealed perinuclear staining pattern which could be seen with other apoptotic nucleases. Irradiation of AEN-expressing cells resulted in synergistic increase of apoptosis whereas AEN deletion mutant in exonuclease domain did not. Our data, thus, suggest that radiation-induced AEN cleaves DNA in concert with other apoptotic nucleases and thereby enhances apoptosis following ionizing irradiation.",

keywords = "Apoptosis, Apoptosis enhancing nuclease, DNase, Ionizing radiation, Radiation response",

author = "Lee, {Ji Hyun} and Koh, {Yeon A.} and Cho, {Chul Koo} and Lee, {Su Jae} and Lee, {Yun Sil} and Sangwoo Bae",

note = "Funding Information: We thank Dr. E. Kim, Dr. Dae Hoon Lee, Sohee Jun, Sunhye Shin, Mira Kim, and Kyung-Joong Kim for advice and technical assistance. We also thank members of laboratory of Radiation Effect at KIRAMS for sharing research information. This work was supported by the Nuclear R&D program from the Ministry of Science and Technology of Korea.",

year = "2005",

month = nov,

day = "11",

doi = "10.1016/j.bbrc.2005.08.264",

language = "English",

volume = "337",

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journal = "Biochemical and Biophysical Research Communications",

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T1 - Identification of a novel ionizing radiation-induced nuclease, AEN, and its functional characterization in apoptosis

AU - Lee, Ji Hyun

AU - Koh, Yeon A.

AU - Cho, Chul Koo

AU - Lee, Su Jae

AU - Lee, Yun Sil

AU - Bae, Sangwoo

N1 - Funding Information: We thank Dr. E. Kim, Dr. Dae Hoon Lee, Sohee Jun, Sunhye Shin, Mira Kim, and Kyung-Joong Kim for advice and technical assistance. We also thank members of laboratory of Radiation Effect at KIRAMS for sharing research information. This work was supported by the Nuclear R&D program from the Ministry of Science and Technology of Korea.

PY - 2005/11/11

Y1 - 2005/11/11

N2 - To investigate ionizing radiation response, we screened genes that exhibit higher expression following γ irradiation. We report here the isolation and functional characterization of a novel ionizing radiation-induced gene, AEN. Sequence analysis of AEN revealed exonuclease domain highly similar to that of exonuclease III. The AEN protein revealed DNase activity by cleaving various DNA substrates. Subcellular distribution of AEN exhibited nuclear colocalization with apoptotic nucleases such as CAD and AIF following irradiation. Moreover AEN distribution revealed perinuclear staining pattern which could be seen with other apoptotic nucleases. Irradiation of AEN-expressing cells resulted in synergistic increase of apoptosis whereas AEN deletion mutant in exonuclease domain did not. Our data, thus, suggest that radiation-induced AEN cleaves DNA in concert with other apoptotic nucleases and thereby enhances apoptosis following ionizing irradiation.

AB - To investigate ionizing radiation response, we screened genes that exhibit higher expression following γ irradiation. We report here the isolation and functional characterization of a novel ionizing radiation-induced gene, AEN. Sequence analysis of AEN revealed exonuclease domain highly similar to that of exonuclease III. The AEN protein revealed DNase activity by cleaving various DNA substrates. Subcellular distribution of AEN exhibited nuclear colocalization with apoptotic nucleases such as CAD and AIF following irradiation. Moreover AEN distribution revealed perinuclear staining pattern which could be seen with other apoptotic nucleases. Irradiation of AEN-expressing cells resulted in synergistic increase of apoptosis whereas AEN deletion mutant in exonuclease domain did not. Our data, thus, suggest that radiation-induced AEN cleaves DNA in concert with other apoptotic nucleases and thereby enhances apoptosis following ionizing irradiation.

KW - Apoptosis

KW - Apoptosis enhancing nuclease

KW - DNase

KW - Ionizing radiation

KW - Radiation response

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AN - SCOPUS:25844451117

SN - 0006-291X

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SP - 39

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JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 1

ER -

Identification of a novel ionizing radiation-induced nuclease, AEN, and its functional characterization in apoptosis

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