Identification and characterization of the Vibrio vulnificus rtxA essential for cytotoxicity in vitro and virulence in mice

Hyun Lee Jeong; Won Kim Myung; Sik Kim Byoung; Min Kim Seung; Cheol Lee Byung; Sung Kim Tae; Ho Choi Sang

Identification and characterization of the Vibrio vulnificus rtxA essential for cytotoxicity in vitro and virulence in mice

Hyun Lee Jeong, Won Kim Myung, Sik Kim Byoung, Min Kim Seung, Cheol Lee Byung, Sung Kim Tae, Ho Choi Sang

Department of Food Science & Biotechnology

Research output: Contribution to journal › Article › peer-review

130 Scopus citations

Abstract

A mutant exhibiting decreased cytotoxic activity toward INT-407 intestinal epithelial cells and carrying a mutation in the rtx gene cluster that consists of rtxCA and rtxBDE operons was screened from a library of V. vulnificus mutants. The functions of the rtxA gene, assessed by constructing an isogenic mutant and evaluating its phenotypic changes, demonstrated that RtxA is essential for the virulence of V. vulnificus in mice as well as in tissue cultures.

Original language	English
Pages (from-to)	146-152
Number of pages	7
Journal	Journal of Microbiology
Volume	45
Issue number	2
State	Published - 2007

Keywords

Cytotoxicity
RtxA
Vibrio vulnificus
Virulence

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title = "Identification and characterization of the Vibrio vulnificus rtxA essential for cytotoxicity in vitro and virulence in mice",

abstract = "A mutant exhibiting decreased cytotoxic activity toward INT-407 intestinal epithelial cells and carrying a mutation in the rtx gene cluster that consists of rtxCA and rtxBDE operons was screened from a library of V. vulnificus mutants. The functions of the rtxA gene, assessed by constructing an isogenic mutant and evaluating its phenotypic changes, demonstrated that RtxA is essential for the virulence of V. vulnificus in mice as well as in tissue cultures.",

keywords = "Cytotoxicity, RtxA, Vibrio vulnificus, Virulence",

author = "Jeong, {Hyun Lee} and Myung, {Won Kim} and Byoung, {Sik Kim} and Seung, {Min Kim} and Byung, {Cheol Lee} and Tae, {Sung Kim} and Sang, {Ho Choi}",

year = "2007",

language = "English",

volume = "45",

pages = "146--152",

journal = "Journal of Microbiology",

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T1 - Identification and characterization of the Vibrio vulnificus rtxA essential for cytotoxicity in vitro and virulence in mice

AU - Jeong, Hyun Lee

AU - Myung, Won Kim

AU - Byoung, Sik Kim

AU - Seung, Min Kim

AU - Byung, Cheol Lee

AU - Tae, Sung Kim

AU - Sang, Ho Choi

PY - 2007

Y1 - 2007

N2 - A mutant exhibiting decreased cytotoxic activity toward INT-407 intestinal epithelial cells and carrying a mutation in the rtx gene cluster that consists of rtxCA and rtxBDE operons was screened from a library of V. vulnificus mutants. The functions of the rtxA gene, assessed by constructing an isogenic mutant and evaluating its phenotypic changes, demonstrated that RtxA is essential for the virulence of V. vulnificus in mice as well as in tissue cultures.

AB - A mutant exhibiting decreased cytotoxic activity toward INT-407 intestinal epithelial cells and carrying a mutation in the rtx gene cluster that consists of rtxCA and rtxBDE operons was screened from a library of V. vulnificus mutants. The functions of the rtxA gene, assessed by constructing an isogenic mutant and evaluating its phenotypic changes, demonstrated that RtxA is essential for the virulence of V. vulnificus in mice as well as in tissue cultures.

KW - Cytotoxicity

KW - RtxA

KW - Vibrio vulnificus

KW - Virulence

UR - http://www.scopus.com/inward/record.url?scp=34248208219&partnerID=8YFLogxK

M3 - Article

C2 - 17483800

AN - SCOPUS:34248208219

SN - 1225-8873

VL - 45

SP - 146

EP - 152

JO - Journal of Microbiology

JF - Journal of Microbiology

IS - 2

ER -

Identification and characterization of the Vibrio vulnificus rtxA essential for cytotoxicity in vitro and virulence in mice

Abstract

Keywords

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