Hydrophobically modified glycol chitosan nanoparticles as carriers for paclitaxel

Jong Ho Kim, Yoo Shin Kim, Sungwon Kim, Jae Hyung Park, Kwangmeyung Kim, Kuiwon Choi, Hesson Chung, Seo Young Jeong, Rang Woon Park, In San Kim, Ick Chan Kwon

Research output: Contribution to journalArticlepeer-review

315 Scopus citations


Self-assembled nanoparticles based on hydrophobically modified glycol chitosan (HGC) were prepared as a carrier for paclitaxel. HGC conjugates were prepared by chemically linking 5β-cholanic acid to glycol chitosan chains using 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide chemistry. In phosphate-buffered saline (PBS; pH 7.4), the synthesized HGC conjugates formed nano-sized particles with a diameter of 200 nm and exhibited high thermodynamic stability as reflected by their low critical aggregation concentration (0.03 mg/ml). Paclitaxel was efficiently loaded into HGC nanoparticles up to 10 wt.% using a dialysis method. The paclitaxel-loaded HGC (PTX-HGC) nanoparticles were 400 nm in diameter and were stable in PBS for 10 days. These PTX-HGC nanoparticles also showed sustained release of the incorporated of paclitaxel (80% of the loaded dose was released in 8 days at 37°C in PBS). Owing to sustained release, the PTX-HGC nanoparticles were less cytotoxic to B16F10 melanoma cells than free paclitaxel formulated in Cremophor EL. Injection of PTX-HGC nanoparticles into the tail vein of tumor-bearing mice prevented increases in tumor volume for 8 days. Finally, PTX was less toxic to the tumor-bearing mice when formulated in HGC nanoparticles than when formulated with Cremophor EL.

Original languageEnglish
Pages (from-to)228-234
Number of pages7
JournalJournal of Controlled Release
Issue number1-2
StatePublished - 10 Mar 2006

Bibliographical note

Funding Information:
This work was supported by the Nanobio project (Intelligent Drug Delivery System) from MOST, the Regional Technology Innovation Program of the MOCIE (no. RTI04-01-01), a grant for Advanced Medical Technology Cluster for Diagnosis and Prediction at Kyungpook National University from MOCIE.


  • Hydrophobically modified glycol chitosan
  • In vivo anti-tumor effect
  • Nanoparticles
  • Paclitaxel


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