Hyaluronic acid induces transglutaminase II to enhance cell motility; role of Rac1 and FAK in the induction of transglutaminase II

Youngmi Kim, Young Woo Park, Yun Sil Lee, Dooil Jeoung

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

The role of transglutaminase II (TGase II) in hyaluronic acid (HA)-promoted melanoma cell motility was investigated. HA induced the expression of TGase II via the nuclear factor κB (NF-kB) in melanoma cells. HA increased the Rac1 activity and phosphorylation of focal adhesion kinase (FAK). Transfection by lipofectamine of dominant-negative Rac1, and FAK-related non-kinase (FRNK), an endogenous inhibitor of FAK, suppressed the induction of TGase II. This suggests that Rac1 and FAK mediate induction of TGase II by HA. HA-promoted melanoma cell motility was inhibited by cystamine, an inhibitor of TGase II, and overexpression of TGase II enhanced melanoma cell motility through reactive oxygen species. Taken together, HA promotes melanoma cell motility through activation of Rac1, FAK, and induction of TGase II.

Original languageEnglish
Pages (from-to)31-39
Number of pages9
JournalBiotechnology Letters
Volume30
Issue number1
DOIs
StatePublished - Jan 2008

Keywords

  • Focal adhesion kinase
  • Hyaluronic acid
  • Motility
  • Rac1
  • Transglutaminase II

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