Hormones and nutrients regulate acetyl-CoA carboxylase promoter I in rat primary hepatocytes

Youn Jung Kim, Mak Soon Lee, Hyun Jung Lee, Yue Wu, Hedley C. Freake, Hyang Sook Chun, Yangha Kim

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


This study investigated the regulation of acetyl-CoA carboxylase (ACC) promoter activity by hormones and nutrients. Genomic clones including promoter I (PI) of the ACC gene were isolated and sequenced. ACC PI fragments (-1,049/+100 or -220/+21 bp) were subcloned into the pGL3-Basic vector that includes luciferase as a reporter gene. The ACC PI/luciferase chimeric plasmids were transfected into primary rat hepatocytes using lipofectin. Insulin treatment increased the activity of -1,049/+100 and -220/+21 ACC PI by 3.0- and 3.5-fold, respectively, compared to the control. The activity of both constructs was also increased by dexamethasone (Dex) and triiodothyronine (T3), with the greatest effects seen with all three hormones present. With -1,049/+100 or -220/+21 ACC PI, the addition of glucose increased luciferase activity compared to glucose-free control (p<0.05). On the other hand, polyunsaturated fatty acids (PUFA) reduced the activity of the -1,049/+100 ACC PI construct, with eicosapentaenoic acid and docosahexaenoic acid showing the greatest effect (about 70% of the control). However, the addition of PUFA to the culture media did not affect the activity of -220/+21 ACC PI. Therefore, insulin, Dex, T 3, glucose, and PUFA regulate ACC gene expression, at least in part, through the PI promoter.

Original languageEnglish
Pages (from-to)124-128
Number of pages5
JournalJournal of Nutritional Science and Vitaminology
Issue number2
StatePublished - Apr 2005


  • Acetyl-CoA carboxylase
  • Gene expression
  • Hormone
  • Nutrition
  • Promoter


Dive into the research topics of 'Hormones and nutrients regulate acetyl-CoA carboxylase promoter I in rat primary hepatocytes'. Together they form a unique fingerprint.

Cite this