Histomorphometric evaluation of onlay freeze-dried block bone and deproteinized bovine bone with collagen in rat

Gyu Un Jung, Seong Jin Hong, Ji Youn Hong, Eun Kyoung Pang

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4 Scopus citations


The aim of this study was to evaluate the effect of human freeze-dried bone block (FDBB) and deproteinized bovine bone with collagen (DBBC) on bone formation when applied as an onlay graft in rat calvariums. Thirty male Sprague-Dawley rats received collagen sponge (control), FDBB, or DBBC onlay grafts trimmed into 8-mm disks measuring 4-mm height. Each graft was secured onto the calvarium surface using horizontal mattress sutures. Rats in each group were killed at 2 (n=5) or 8 (n=5) weeks postoperatively for histologic and histomorphometric analysis. The total augmented area (mm2), new bone area (mm2), and bone density (%) were measured. The FDBB and DBBC groups showed significantly more new bone formation and bone density than the control group at 2 and 8 weeks. The increased new bone area was significantly greater in the FDBB group than in the DBBC group (p<0.05). The total augmented area was significantly higher in the FDBB and DBBC groups at 2 and 8 weeks than in the control group (p<0.05), and at 8 weeks, the area was significantly decreased in the DBBC group compared to that in the FDBB group and the area at 2 weeks (p<0.05). Within the limitations of the present study, we concluded that onlay FDBB and DBBC grafts caused new bone formation through an osteoconductive mechanism. In addition, compared to FDBB, DBBC had less capacity to form new bone and maintain the space.

Original languageEnglish
Pages (from-to)70-77
Number of pages8
JournalTissue Engineering and Regenerative Medicine
Issue number1
StatePublished - 1 Feb 2016

Bibliographical note

Publisher Copyright:
© 2016, The Korean Tissue Engineering and Regenerative Medicine Society and Springer Science+Business Media Dordrecht.


  • Bone formation
  • Deproteinized bovine bone with collagen
  • Human freeze dried corticocancellous bone block
  • Onlay graft
  • Space maintenance


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