Highly Sensitive Detection of Bisphenol A by NanoAptamer Assay with Truncated Aptamer

Eun Hee Lee, Hyun Jeong Lim, Sang Don Lee, Ahjeong Son

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76 Scopus citations


For the sensitive quantification of bisphenol A (BPA), we have developed NanoAptamer assay, which employs aptamer and complementary signaling DNA, a set of quantum dots (QD), and magnetic beads (MBs). Signaling DNA-QD655 was tethered to MB-QD565 via the aptamer. The affinity of the aptamer to BPA resulted in the release of the signaling DNA-QD655 from the complex and hence the corresponding decrease in the QD655 fluorescence measurement signal. Three new aptamers (23, 58, and 24-mer) were designed via truncation of the reference aptamer (73-mer). The sensitivity and selectivity of each aptamer for BPA detection via NanoAptamer assay were investigated. One of the truncated aptamers (24-mer) has shown a significantly better performance (limit of detection, LOD, 0.17 pg/mL) than the reference 73-mer aptamer (LOD, 570 pg/mL). It has also shown the best selectivity for BPA detection over BPA analogues (i.e., bisphenol B, bisphenol C, and diethylstilbestrol). It corresponded to a normalized fluorescence change of 33.7% at the environmentally relevant concentration of 1 ng/mL (1 ppb) BPA; however, the analogues remained unchanged (2.3-3.9%).

Original languageEnglish
Pages (from-to)14889-14898
Number of pages10
JournalACS Applied Materials and Interfaces
Issue number17
StatePublished - 3 May 2017

Bibliographical note

Publisher Copyright:
© 2017 American Chemical Society.


  • bisphenol A
  • endocrine-disrupting chemicals
  • NanoAptamer assay
  • quantum dots
  • truncated aptamer


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