Highly Selective and Sensitive Two-Photon Fluorescence Probe for Endogenous Peroxynitrite Detection and Its Applications in Living Cells and Tissues

Jun Li; Chang Su Lim; Gyoungmi Kim; Hwan Myung Kim; Juyoung Yoon

doi:10.1021/acs.analchem.7b02059

Highly Selective and Sensitive Two-Photon Fluorescence Probe for Endogenous Peroxynitrite Detection and Its Applications in Living Cells and Tissues

Jun Li, Chang Su Lim, Gyoungmi Kim, Hwan Myung Kim, Juyoung Yoon

Chemistry & Nanoscience

Research output: Contribution to journal › Article › peer-review

92 Scopus citations

Abstract

A new two-photon fluorescence probe for endogenous peroxynitrite (ONOO^-) detection was designed and synthesized. The probe exhibits good selectivity and sensitivity for ONOO^- in phosphate-buffered saline solution with a low detection limit (3.5 × 10^-8 M). Furthermore, the probe displays good performance in detecting endogenous ONOO^-, not only in RAW 264.7 cells but also in rat hippocampal tissue, with a high two-photon cross-section value (δ ≈ 100 GM) at a deep depth of 120 μm.

Original language	English
Pages (from-to)	8496-8500
Number of pages	5
Journal	Analytical Chemistry
Volume	89
Issue number	16
DOIs	https://doi.org/10.1021/acs.analchem.7b02059
State	Published - 15 Aug 2017

Bibliographical note

Funding Information:
This study was supported by grants from the National Creative Research Initiative programs of the National Research Foundation of Korea (NRF) funded by the Korean government (MSIP) (No. 2012R1A3A2048814) and the National Leading Research Lab Program (No. 2016R1E1A1A02920873). Mass spectral data were obtained from the Korea Basic Science Institute (Daegu) using a Jeol JMS 700 high-resolution mass spectrometer.

Publisher Copyright:
© 2017 American Chemical Society.

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title = "Highly Selective and Sensitive Two-Photon Fluorescence Probe for Endogenous Peroxynitrite Detection and Its Applications in Living Cells and Tissues",

abstract = "A new two-photon fluorescence probe for endogenous peroxynitrite (ONOO-) detection was designed and synthesized. The probe exhibits good selectivity and sensitivity for ONOO- in phosphate-buffered saline solution with a low detection limit (3.5 × 10-8 M). Furthermore, the probe displays good performance in detecting endogenous ONOO-, not only in RAW 264.7 cells but also in rat hippocampal tissue, with a high two-photon cross-section value (δ ≈ 100 GM) at a deep depth of 120 μm.",

author = "Jun Li and Lim, {Chang Su} and Gyoungmi Kim and Kim, {Hwan Myung} and Juyoung Yoon",

note = "Funding Information: This study was supported by grants from the National Creative Research Initiative programs of the National Research Foundation of Korea (NRF) funded by the Korean government (MSIP) (No. 2012R1A3A2048814) and the National Leading Research Lab Program (No. 2016R1E1A1A02920873). Mass spectral data were obtained from the Korea Basic Science Institute (Daegu) using a Jeol JMS 700 high-resolution mass spectrometer. Publisher Copyright: {\textcopyright} 2017 American Chemical Society.",

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doi = "10.1021/acs.analchem.7b02059",

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AU - Lim, Chang Su

AU - Kim, Gyoungmi

AU - Kim, Hwan Myung

AU - Yoon, Juyoung

N1 - Funding Information: This study was supported by grants from the National Creative Research Initiative programs of the National Research Foundation of Korea (NRF) funded by the Korean government (MSIP) (No. 2012R1A3A2048814) and the National Leading Research Lab Program (No. 2016R1E1A1A02920873). Mass spectral data were obtained from the Korea Basic Science Institute (Daegu) using a Jeol JMS 700 high-resolution mass spectrometer. Publisher Copyright: © 2017 American Chemical Society.

PY - 2017/8/15

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N2 - A new two-photon fluorescence probe for endogenous peroxynitrite (ONOO-) detection was designed and synthesized. The probe exhibits good selectivity and sensitivity for ONOO- in phosphate-buffered saline solution with a low detection limit (3.5 × 10-8 M). Furthermore, the probe displays good performance in detecting endogenous ONOO-, not only in RAW 264.7 cells but also in rat hippocampal tissue, with a high two-photon cross-section value (δ ≈ 100 GM) at a deep depth of 120 μm.

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Highly Selective and Sensitive Two-Photon Fluorescence Probe for Endogenous Peroxynitrite Detection and Its Applications in Living Cells and Tissues

Abstract

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