Highly Selective and Sensitive Two-Photon Fluorescence Probe for Endogenous Peroxynitrite Detection and Its Applications in Living Cells and Tissues

Jun Li, Chang Su Lim, Gyoungmi Kim, Hwan Myung Kim, Juyoung Yoon

Research output: Contribution to journalArticlepeer-review

95 Scopus citations

Abstract

A new two-photon fluorescence probe for endogenous peroxynitrite (ONOO-) detection was designed and synthesized. The probe exhibits good selectivity and sensitivity for ONOO- in phosphate-buffered saline solution with a low detection limit (3.5 × 10-8 M). Furthermore, the probe displays good performance in detecting endogenous ONOO-, not only in RAW 264.7 cells but also in rat hippocampal tissue, with a high two-photon cross-section value (δ ≈ 100 GM) at a deep depth of 120 μm.

Original languageEnglish
Pages (from-to)8496-8500
Number of pages5
JournalAnalytical Chemistry
Volume89
Issue number16
DOIs
StatePublished - 15 Aug 2017

Bibliographical note

Funding Information:
This study was supported by grants from the National Creative Research Initiative programs of the National Research Foundation of Korea (NRF) funded by the Korean government (MSIP) (No. 2012R1A3A2048814) and the National Leading Research Lab Program (No. 2016R1E1A1A02920873). Mass spectral data were obtained from the Korea Basic Science Institute (Daegu) using a Jeol JMS 700 high-resolution mass spectrometer.

Publisher Copyright:
© 2017 American Chemical Society.

Fingerprint

Dive into the research topics of 'Highly Selective and Sensitive Two-Photon Fluorescence Probe for Endogenous Peroxynitrite Detection and Its Applications in Living Cells and Tissues'. Together they form a unique fingerprint.

Cite this