Highly Selective and Sensitive Two-Photon Fluorescence Probe for Endogenous Peroxynitrite Detection and Its Applications in Living Cells and Tissues

Jun Li, Chang Su Lim, Gyoungmi Kim, Hwan Myung Kim, Juyoung Yoon

Research output: Contribution to journalArticlepeer-review

81 Scopus citations

Abstract

A new two-photon fluorescence probe for endogenous peroxynitrite (ONOO-) detection was designed and synthesized. The probe exhibits good selectivity and sensitivity for ONOO- in phosphate-buffered saline solution with a low detection limit (3.5 × 10-8 M). Furthermore, the probe displays good performance in detecting endogenous ONOO-, not only in RAW 264.7 cells but also in rat hippocampal tissue, with a high two-photon cross-section value (δ ≈ 100 GM) at a deep depth of 120 μm.

Original languageEnglish
Pages (from-to)8496-8500
Number of pages5
JournalAnalytical Chemistry
Volume89
Issue number16
DOIs
StatePublished - 15 Aug 2017

Fingerprint

Dive into the research topics of 'Highly Selective and Sensitive Two-Photon Fluorescence Probe for Endogenous Peroxynitrite Detection and Its Applications in Living Cells and Tissues'. Together they form a unique fingerprint.

Cite this