Collagen is the most prevalent scaffold material for in vitro skin models. The major limitation of collagen scaffold is its mechanical weakness, resulting in severe contraction during differentiation. Here, we presented a slime-webbed scaffold composed of perpendicularly stacked fibers with large pores. This slime-webbed scaffold did not contract while improving molecular transport and achieving comparable cell viability. Fibroblasts were seeded into the slime-webbed scaffold to mimic the dermal layer. In the epidermal layer, which was on top of this scaffold, keratinocytes expressed the differentiation biomarkers, keratin-5 and involucrin. Our slime-webbed scaffold-based human skin models overcome the critical limitations of collagen scaffold, suggesting a promising alternative skin model for consistent testing of drugs or cosmetic products.
Bibliographical noteFunding Information:
This work was supported in part by the Technology development Program of MSS (S3251721, S3316767) by Korea Technology and Information Promotion Agency, Basic Research Lab (2022R1A4A2000748) and Bio & Medical Technology Development Program (2022M3A9B6018217) by National Research Foundation of Korea, Technology Innovation Program (20008414) and the Alchemist Project of the Korea Evaluation Institute of Industrial Technology (KEIT 20018560, NTIS 1415180625) by the Ministry of Trade, Industry & Energy (MOTIE), Hongik University Research Fund. It was also supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) by the Ministry of Education, Science and Technology (NRF-2021R1F1A1056188), the High Value-added Food Technology Development Program, the Ministry of Agriculture, Food and Rural Affairs (MAFRA) (321021031HD030), RP-Grant 2020 and Research Grant 2020–2021 of Ewha Womans University.
© 2023, The Korean Society for Biotechnology and Bioengineering and Springer.
- human skin model
- slime-webbing method