Highly efficient protein expression and purification using bacterial hemoglobin fusion vector

Soo Young Kwon, Yoon Joo Choi, Tae Hong Kang, Kwang Hoon Lee, Sun Shin Cha, Gyung Hwa Kim, Heung Soo Lee, Kyong Tai Kim, Kyung Jin Kim

Research output: Contribution to journalArticlepeer-review

33 Scopus citations

Abstract

Recently developed bacterial hemoglobin (VHb) fusion expression vector has been widely used for the production of many target proteins due to its distinctive properties of expressing fusion protein with red color which facilitates visualization of the steps in purification, and increasing solubility of the target proteins. However, after intensive use of the vector, several defects have been found. In this report, we present a modified VHb fusion vector (pPosKJ) with higher efficiency, in which most of the defects were eliminated. First, it was found that thrombin protease often digests target protein as well as inserted thrombin cleavage site, so it was replaced by a TEV cleavage site for more specific cleavage of VHb from target protein. Second, a glycine-rich linker sequence was inserted between 6× his-tag and VHb to improve the affinity of 6× his-tag to Ni-NTA resin, resulting in higher purity of eluted fusion protein. Third, EcoRI and XhoI restriction sites located elsewhere in the vector were removed to make these restriction sites available for the cloning of target protein coding genes. A pPosKJ vector was fully examined with an anti-apoptotic BCL-2 family member of Caenorhabditis elegans, CED-9. A C-terminal VHb fusion expression vector (pPosKJC) was also constructed for stable expression of target proteins that may be difficult to express with an N-terminal fusion. Vaccinia-related kinase 1 (VRK1) was also successfully expressed and purified using the vector with high yield. Taken together, we suggest that the VHb fusion vector may be well suited for high-throughput protein expression and purification.

Original languageEnglish
Pages (from-to)274-282
Number of pages9
JournalPlasmid
Volume53
Issue number3
DOIs
StatePublished - May 2005

Keywords

  • Bacterial hemoglobin
  • CED-9
  • Fusion expression vector
  • Vaccinia-related kinase 1
  • Vitreoscilla

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