Higher DNA repair activity is related with longer replicative life span in mammalian embryonic fibroblast cells

Seong Hoon Park; Hong Jun Kang; Hyun Seok Kim; Min Ju Kim; Jee In Heo; Jeong Hyeon Kim; Yoon Jung Kho; Sung Chan Kim; Jaebong Kim; Jae Bong Park; Jae Yong Lee

doi:10.1007/s10522-011-9355-2

Higher DNA repair activity is related with longer replicative life span in mammalian embryonic fibroblast cells

Seong Hoon Park, Hong Jun Kang, Hyun Seok Kim, Min Ju Kim, Jee In Heo, Jeong Hyeon Kim, Yoon Jung Kho, Sung Chan Kim, Jaebong Kim, Jae Bong Park, Jae Yong Lee

Department of Life Science

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Since the detailed comparison of DNA repair activities among mammalian embryonic fibroblast cells with different replicative life spans has not been investigated, we tested DNA repair activities in embryonic fibroblast cells derived from mammals including human, dog, rat, and mouse. The cell viability after treatment of four DNA damage agents appeared to be decreased in the order of human embryonic fibroblasts (HEFs) > dog embryonic fibroblasts (DEFs) > rat embryonic fibroblasts (REFs) > mouse embryonic fibroblasts (MEFs) although statistical significance was lacking. The amounts of strand breaks and AP (apurinic/apyrimidinic) sites also appear to be decreased in the order of HEFs > DEFs > REFs ≥ MEFs after treatment of DNA damage agents. The DNA repair activities and rates including base excision repair (BER), nucleotide excision repair (NER) and double-strand break repair (DSBR) including non-homologous end-joining (NHEJ) decreased again in the order of HEFs > DEFs > REFs ≥ MEFs. BER and NHEJ activities in 3% O ₂ also decreased in the order of HEFs > DEFs > REFs > MEFs. This order in DNA repair activity appears to be coincident with that of replicative life span of fibroblasts and that of life span of mammals. These results indicate that higher DNA repair activity is related with longer replicative life span in embryonic fibroblast cells.

Original language	English
Pages (from-to)	565-579
Number of pages	15
Journal	Biogerontology
Volume	12
Issue number	6
DOIs	https://doi.org/10.1007/s10522-011-9355-2
State	Published - Dec 2011

Bibliographical note

Funding Information:
Acknowledgments This work was supported by Priority Research Centers Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2010-0029642).

Keywords

DNA damage
DNA repair
DNA repair rate
Mammalian embryonic fibroblasts
Replicative life span

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10.1007/s10522-011-9355-2

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title = "Higher DNA repair activity is related with longer replicative life span in mammalian embryonic fibroblast cells",

abstract = "Since the detailed comparison of DNA repair activities among mammalian embryonic fibroblast cells with different replicative life spans has not been investigated, we tested DNA repair activities in embryonic fibroblast cells derived from mammals including human, dog, rat, and mouse. The cell viability after treatment of four DNA damage agents appeared to be decreased in the order of human embryonic fibroblasts (HEFs) > dog embryonic fibroblasts (DEFs) > rat embryonic fibroblasts (REFs) > mouse embryonic fibroblasts (MEFs) although statistical significance was lacking. The amounts of strand breaks and AP (apurinic/apyrimidinic) sites also appear to be decreased in the order of HEFs > DEFs > REFs ≥ MEFs after treatment of DNA damage agents. The DNA repair activities and rates including base excision repair (BER), nucleotide excision repair (NER) and double-strand break repair (DSBR) including non-homologous end-joining (NHEJ) decreased again in the order of HEFs > DEFs > REFs ≥ MEFs. BER and NHEJ activities in 3% O 2 also decreased in the order of HEFs > DEFs > REFs > MEFs. This order in DNA repair activity appears to be coincident with that of replicative life span of fibroblasts and that of life span of mammals. These results indicate that higher DNA repair activity is related with longer replicative life span in embryonic fibroblast cells.",

keywords = "DNA damage, DNA repair, DNA repair rate, Mammalian embryonic fibroblasts, Replicative life span",

author = "Park, {Seong Hoon} and Kang, {Hong Jun} and Kim, {Hyun Seok} and Kim, {Min Ju} and Heo, {Jee In} and Kim, {Jeong Hyeon} and Kho, {Yoon Jung} and Kim, {Sung Chan} and Jaebong Kim and Park, {Jae Bong} and Lee, {Jae Yong}",

note = "Funding Information: Acknowledgments This work was supported by Priority Research Centers Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2010-0029642).",

year = "2011",

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doi = "10.1007/s10522-011-9355-2",

language = "English",

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AU - Park, Seong Hoon

AU - Kang, Hong Jun

AU - Kim, Hyun Seok

AU - Kim, Min Ju

AU - Heo, Jee In

AU - Kim, Jeong Hyeon

AU - Kho, Yoon Jung

AU - Kim, Sung Chan

AU - Kim, Jaebong

AU - Park, Jae Bong

AU - Lee, Jae Yong

N1 - Funding Information: Acknowledgments This work was supported by Priority Research Centers Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2010-0029642).

PY - 2011/12

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N2 - Since the detailed comparison of DNA repair activities among mammalian embryonic fibroblast cells with different replicative life spans has not been investigated, we tested DNA repair activities in embryonic fibroblast cells derived from mammals including human, dog, rat, and mouse. The cell viability after treatment of four DNA damage agents appeared to be decreased in the order of human embryonic fibroblasts (HEFs) > dog embryonic fibroblasts (DEFs) > rat embryonic fibroblasts (REFs) > mouse embryonic fibroblasts (MEFs) although statistical significance was lacking. The amounts of strand breaks and AP (apurinic/apyrimidinic) sites also appear to be decreased in the order of HEFs > DEFs > REFs ≥ MEFs after treatment of DNA damage agents. The DNA repair activities and rates including base excision repair (BER), nucleotide excision repair (NER) and double-strand break repair (DSBR) including non-homologous end-joining (NHEJ) decreased again in the order of HEFs > DEFs > REFs ≥ MEFs. BER and NHEJ activities in 3% O 2 also decreased in the order of HEFs > DEFs > REFs > MEFs. This order in DNA repair activity appears to be coincident with that of replicative life span of fibroblasts and that of life span of mammals. These results indicate that higher DNA repair activity is related with longer replicative life span in embryonic fibroblast cells.

AB - Since the detailed comparison of DNA repair activities among mammalian embryonic fibroblast cells with different replicative life spans has not been investigated, we tested DNA repair activities in embryonic fibroblast cells derived from mammals including human, dog, rat, and mouse. The cell viability after treatment of four DNA damage agents appeared to be decreased in the order of human embryonic fibroblasts (HEFs) > dog embryonic fibroblasts (DEFs) > rat embryonic fibroblasts (REFs) > mouse embryonic fibroblasts (MEFs) although statistical significance was lacking. The amounts of strand breaks and AP (apurinic/apyrimidinic) sites also appear to be decreased in the order of HEFs > DEFs > REFs ≥ MEFs after treatment of DNA damage agents. The DNA repair activities and rates including base excision repair (BER), nucleotide excision repair (NER) and double-strand break repair (DSBR) including non-homologous end-joining (NHEJ) decreased again in the order of HEFs > DEFs > REFs ≥ MEFs. BER and NHEJ activities in 3% O 2 also decreased in the order of HEFs > DEFs > REFs > MEFs. This order in DNA repair activity appears to be coincident with that of replicative life span of fibroblasts and that of life span of mammals. These results indicate that higher DNA repair activity is related with longer replicative life span in embryonic fibroblast cells.

KW - DNA damage

KW - DNA repair

KW - DNA repair rate

KW - Mammalian embryonic fibroblasts

KW - Replicative life span

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ER -

Higher DNA repair activity is related with longer replicative life span in mammalian embryonic fibroblast cells

Abstract

Bibliographical note

Keywords

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