High-Resolution Profiling of Histone Methylations in the Human Genome

Artem Barski; Suresh Cuddapah; Kairong Cui; Tae Young Roh; Dustin E. Schones; Zhibin Wang; Gang Wei; Iouri Chepelev; Keji Zhao

doi:10.1016/j.cell.2007.05.009

High-Resolution Profiling of Histone Methylations in the Human Genome

Artem Barski, Suresh Cuddapah, Kairong Cui, Tae Young Roh, Dustin E. Schones, Zhibin Wang, Gang Wei, Iouri Chepelev, Keji Zhao

Department of Life Science

Research output: Contribution to journal › Article › peer-review

5485 Scopus citations

Abstract

Histone modifications are implicated in influencing gene expression. We have generated high-resolution maps for the genome-wide distribution of 20 histone lysine and arginine methylations as well as histone variant H2A.Z, RNA polymerase II, and the insulator binding protein CTCF across the human genome using the Solexa 1G sequencing technology. Typical patterns of histone methylations exhibited at promoters, insulators, enhancers, and transcribed regions are identified. The monomethylations of H3K27, H3K9, H4K20, H3K79, and H2BK5 are all linked to gene activation, whereas trimethylations of H3K27, H3K9, and H3K79 are linked to repression. H2A.Z associates with functional regulatory elements, and CTCF marks boundaries of histone methylation domains. Chromosome banding patterns are correlated with unique patterns of histone modifications. Chromosome breakpoints detected in T cell cancers frequently reside in chromatin regions associated with H3K4 methylations. Our data provide new insights into the function of histone methylation and chromatin organization in genome function.

Original language	English
Pages (from-to)	823-837
Number of pages	15
Journal	Cell
Volume	129
Issue number	4
DOIs	https://doi.org/10.1016/j.cell.2007.05.009
State	Published - 18 May 2007

Bibliographical note

Funding Information:
We thank Dr. Warren Leonard for critical reading of the manuscript. This work was supported by the Intramural Research Program of the NIH, National Heart, Lung, and Blood Institute.

Keywords

DNA
PROTEINS
SYSBIO

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.cell.2007.05.009

Cite this

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title = "High-Resolution Profiling of Histone Methylations in the Human Genome",

abstract = "Histone modifications are implicated in influencing gene expression. We have generated high-resolution maps for the genome-wide distribution of 20 histone lysine and arginine methylations as well as histone variant H2A.Z, RNA polymerase II, and the insulator binding protein CTCF across the human genome using the Solexa 1G sequencing technology. Typical patterns of histone methylations exhibited at promoters, insulators, enhancers, and transcribed regions are identified. The monomethylations of H3K27, H3K9, H4K20, H3K79, and H2BK5 are all linked to gene activation, whereas trimethylations of H3K27, H3K9, and H3K79 are linked to repression. H2A.Z associates with functional regulatory elements, and CTCF marks boundaries of histone methylation domains. Chromosome banding patterns are correlated with unique patterns of histone modifications. Chromosome breakpoints detected in T cell cancers frequently reside in chromatin regions associated with H3K4 methylations. Our data provide new insights into the function of histone methylation and chromatin organization in genome function.",

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author = "Artem Barski and Suresh Cuddapah and Kairong Cui and Roh, {Tae Young} and Schones, {Dustin E.} and Zhibin Wang and Gang Wei and Iouri Chepelev and Keji Zhao",

note = "Funding Information: We thank Dr. Warren Leonard for critical reading of the manuscript. This work was supported by the Intramural Research Program of the NIH, National Heart, Lung, and Blood Institute. ",

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AU - Barski, Artem

AU - Cuddapah, Suresh

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AU - Schones, Dustin E.

AU - Wang, Zhibin

AU - Wei, Gang

AU - Chepelev, Iouri

AU - Zhao, Keji

N1 - Funding Information: We thank Dr. Warren Leonard for critical reading of the manuscript. This work was supported by the Intramural Research Program of the NIH, National Heart, Lung, and Blood Institute.

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High-Resolution Profiling of Histone Methylations in the Human Genome

Abstract

Bibliographical note

Keywords

UN SDGs

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