High-density spheroid arrays for 3-D liver cell culture and secretion analysis

Mimi Y. Zhang, Dino Di Carlo, Liz Y. Wu, Luke P. Lee

Research output: Contribution to conferencePaperpeer-review

Abstract

To effectively coalesce otherwise monolayer liver cells (hepatocytes) seeded into microfluidic channels, we present a high-density cell-trapping array chip for rapid three-dimensional hepatocyte spheroid self-assembly. Moreover, by modifying trap geometries, spheroids of varying sizes can be formed in order to optimize the spatial microenvironment for improving cell viability and function. Preliminary sensitivity analysis of albumin secretion detection demonstrates the feasibility of using an optimized chemiluminescent immunoassay to determine the albumin concentrations in the range of 1 ng/mL to 500 ng/mL.

Original languageEnglish
Pages1477-1479
Number of pages3
StatePublished - 2006
Event10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 - Tokyo, Japan
Duration: 5 Nov 20069 Nov 2006

Conference

Conference10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006
Country/TerritoryJapan
CityTokyo
Period5/11/069/11/06

Keywords

  • Cell culture array
  • Cell-trapping
  • Hepatocyte
  • Spheroid

Fingerprint

Dive into the research topics of 'High-density spheroid arrays for 3-D liver cell culture and secretion analysis'. Together they form a unique fingerprint.

Cite this