Abstract
To effectively coalesce otherwise monolayer liver cells (hepatocytes) seeded into microfluidic channels, we present a high-density cell-trapping array chip for rapid three-dimensional hepatocyte spheroid self-assembly. Moreover, by modifying trap geometries, spheroids of varying sizes can be formed in order to optimize the spatial microenvironment for improving cell viability and function. Preliminary sensitivity analysis of albumin secretion detection demonstrates the feasibility of using an optimized chemiluminescent immunoassay to determine the albumin concentrations in the range of 1 ng/mL to 500 ng/mL.
Original language | English |
---|---|
Pages | 1477-1479 |
Number of pages | 3 |
State | Published - 2006 |
Event | 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 - Tokyo, Japan Duration: 5 Nov 2006 → 9 Nov 2006 |
Conference
Conference | 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 |
---|---|
Country/Territory | Japan |
City | Tokyo |
Period | 5/11/06 → 9/11/06 |
Keywords
- Cell culture array
- Cell-trapping
- Hepatocyte
- Spheroid