Hepatoprotective effects of oyster hydrolysate on lipopolysaccharide/D-galactosamine-induced acute liver injury in mice

Ji Hyeon Ryu, Eun Jin Kim, Chengliang Xie, Marie Merci Nyiramana, Adrian S. Siregar, Si Hyang Park, Soo Buem Cho, Dae Hyun Song, Nam Gil Kim, Yeung Joon Choi, Sang Soo Kang, Dawon Kang

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3 Scopus citations


Oxidative stress and inflammation are key factors responsible for progression of liver injury. A variety of functions of oyster hydrolysate (OH) are affected by their antioxidant and anti-inflammatory activities. However, little is known regarding the effects of OH on a liver injury model. This study was performed to evaluate the effects of OH on acute liver injury induced by lipopolysaccharide/D-galactosamine (LPS/D-GalN) in mice. Experimental groups were divided into six groups as follows (each group, n=10): control (saline), LPS/D-GalN, LPS/D-GalN+OH (100 mg/kg), LPS/D-GalN+OH (200 mg/kg), LPS/D-GalN+OH (400 mg/kg), and LPS/D-GalN+silymarin (25 mg/kg, positive control). The experimental acute liver injury model was induced with LPS (1 μg/kg) and D-GalN (400 mg/kg). We first analyzed antioxidant and anti-inflammatory activities in OH. OH showed high DPPH and ABTS radical scavenging activities and reduced ROS generation in Chang cells in a dose-dependent manner. In addition, OH showed anti-inflammatory activities, such as inhibition of cyclooxygenase-2 and 5-lipooxygenase. Treatment with OH down-regulated tumor necrosis factor (TNF)-α, interleukin (IL)-6, and IL-1α expression levels in LPS-stimulated RAW264.7 cells. OH significantly reduced LPS/D-GalN-induced increases in the concentrations of alanine transaminase and aspartate aminotransferase in serum. In the LPS/D-GalN group, liver tissues exhibited apoptosis of hepatocytes with hemorrhages. These pathological alterations were ameliorated by OH treatment. Consistently, hepatic catalase activity was low in the LPS/D-GalN group compared to the control group, and catalase activity was significantly restored by OH treatment (P<0.05). Furthermore, OH markedly reduced the LPS/D-GalN-induced increase in TNF-α, IL-1β, and IL-6 levels in liver tissue. Taken together, these results show that OH has hepatoprotective effects on LPS/D-GalN-induced acute liver injury via inhibition of oxidative stress and inflammation, suggesting that OH could be used as a health functional food and potential therapeutic agent for acute liver injury.

Original languageEnglish
Pages (from-to)659-670
Number of pages12
JournalJournal of the Korean Society of Food Science and Nutrition
Issue number6
StatePublished - Jun 2017

Bibliographical note

Publisher Copyright:
© 2017, Korean Society of Food Science and Nutrition. All rights reserved.


  • Acute liver injury
  • Anti-inflammatory
  • Antioxidant
  • Apoptosis
  • Oyster hydrolysate


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