Abstract
To investigate the effect of receptor substrate of target cells on stem cell differentiation, lactobionic acid-conjugated poly[(propylene glycol)-b-(ethylene glycol)-b-(propylene glycol)]-poly(l-alanine) (LB-PLX-PA) was synthesized, and then thermogelling systems consisting of LB-PLX-PA and PLX-PA in a ratio of 0/100 (LB-0), 5/95 (LB-5), and 20/80 (LB-20) were constructed as an injectable three-dimensional scaffold toward hepatogenic differentiation of tonsil-derived mesenchymal stem cells (TMSCs). Modulus of LB-0, LB-5, and LB-20 increased to 500-800 Pa at 37 °C (gel) due to the heat induced sol-to-gel transition of the systems during which TMSCs were incorporated into the gel. Based on biomarker expressions and hepatic biofunctions of the differentiated cells, the receptor substrate (LB)-conjugated bioactive thermogel provides compatible microenvironments for the differentiated cells, and thus gives pronounced positive results on the differentiation of the stem cells into target cells during three-dimensional culture, compared with a passive thermogel.
| Original language | English |
|---|---|
| Pages (from-to) | 1305-1309 |
| Number of pages | 5 |
| Journal | ACS Macro Letters |
| Volume | 6 |
| Issue number | 11 |
| DOIs | |
| State | Published - 21 Nov 2017 |
Bibliographical note
Funding Information:This work was supported by the National Research Foundation of Korea Grant funded by the Korean Government (2012M3A9C6049835, 2017R1A2B2007365, 2017R1A5A1015365).
Publisher Copyright:
© 2017 American Chemical Society.