Hepatitis B virus X protein modulates peroxisome proliferator-activated receptor γ through protein-protein interaction

Youn Hee Choi; Ha Il Kim; Je Kyung Seong; Dae Yeul Yu; Hyeseong Cho; Mi Ock Lee; Jae Myun Lee; Yong Ho Ahn; Se Jong Kim; Jeon Han Park

doi:10.1016/S0014-5793(03)01449-2

Hepatitis B virus X protein modulates peroxisome proliferator-activated receptor γ through protein-protein interaction

Youn Hee Choi, Ha Il Kim, Je Kyung Seong, Dae Yeul Yu, Hyeseong Cho, Mi Ock Lee, Jae Myun Lee, Yong Ho Ahn, Se Jong Kim, Jeon Han Park

Department of Physiology

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

Ligand activation of peroxisome proliferator-activated receptor γ (PPARγ) has been reported to induce growth inhibition and apoptosis in various cancers including hepatocellular carcinoma (HCC). However, the effect of hepatitis B virus X protein (HBx) on PPARγ activation has not been characterized in hepatitis B virus (HBV)-associated HCC. Herein, we demonstrated that HBx counteracted growth inhibition caused by PPARγ ligand in HBx-associated HCC cells. We found that HBx bound to DNA binding domain of PPARγ and HBx/PPARγ interaction blocked nuclear localization and binding to recognition site of PPARγ. HBx significantly suppressed a PPARγ-mediated transactivation. These results suggest that HBx modulates PPARγ function through protein-protein interaction.

Original language	English
Pages (from-to)	73-80
Number of pages	8
Journal	FEBS Letters
Volume	557
Issue number	1-3
DOIs	https://doi.org/10.1016/S0014-5793(03)01449-2
State	Published - 16 Jan 2004

Bibliographical note

Funding Information:
We thank Drs. Ronald M. Evans, David J. Mangelsdof, and Heonjoong Kang for providing us with PPRE 3 -tk-LUC and pCMX-mPPARγ and Sankyo Co. Ltd for the troglitazone. This work was supported by a grant from the Korean National Cancer Control Program, Ministry of Health and Welfare, South Korea (02-1-2-0590).

Keywords

Hepatitis B virus X protein
Peroxisome proliferator-activated receptor γ
Protein-protein interaction
Transactivation

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10.1016/S0014-5793(03)01449-2

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title = "Hepatitis B virus X protein modulates peroxisome proliferator-activated receptor γ through protein-protein interaction",

abstract = "Ligand activation of peroxisome proliferator-activated receptor γ (PPARγ) has been reported to induce growth inhibition and apoptosis in various cancers including hepatocellular carcinoma (HCC). However, the effect of hepatitis B virus X protein (HBx) on PPARγ activation has not been characterized in hepatitis B virus (HBV)-associated HCC. Herein, we demonstrated that HBx counteracted growth inhibition caused by PPARγ ligand in HBx-associated HCC cells. We found that HBx bound to DNA binding domain of PPARγ and HBx/PPARγ interaction blocked nuclear localization and binding to recognition site of PPARγ. HBx significantly suppressed a PPARγ-mediated transactivation. These results suggest that HBx modulates PPARγ function through protein-protein interaction.",

keywords = "Hepatitis B virus X protein, Peroxisome proliferator-activated receptor γ, Protein-protein interaction, Transactivation",

author = "Choi, {Youn Hee} and Kim, {Ha Il} and Seong, {Je Kyung} and Yu, {Dae Yeul} and Hyeseong Cho and Lee, {Mi Ock} and Lee, {Jae Myun} and Ahn, {Yong Ho} and Kim, {Se Jong} and Park, {Jeon Han}",

note = "Funding Information: We thank Drs. Ronald M. Evans, David J. Mangelsdof, and Heonjoong Kang for providing us with PPRE 3 -tk-LUC and pCMX-mPPARγ and Sankyo Co. Ltd for the troglitazone. This work was supported by a grant from the Korean National Cancer Control Program, Ministry of Health and Welfare, South Korea (02-1-2-0590).",

year = "2004",

month = jan,

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doi = "10.1016/S0014-5793(03)01449-2",

language = "English",

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T1 - Hepatitis B virus X protein modulates peroxisome proliferator-activated receptor γ through protein-protein interaction

AU - Choi, Youn Hee

AU - Kim, Ha Il

AU - Seong, Je Kyung

AU - Yu, Dae Yeul

AU - Cho, Hyeseong

AU - Lee, Mi Ock

AU - Lee, Jae Myun

AU - Ahn, Yong Ho

AU - Kim, Se Jong

AU - Park, Jeon Han

N1 - Funding Information: We thank Drs. Ronald M. Evans, David J. Mangelsdof, and Heonjoong Kang for providing us with PPRE 3 -tk-LUC and pCMX-mPPARγ and Sankyo Co. Ltd for the troglitazone. This work was supported by a grant from the Korean National Cancer Control Program, Ministry of Health and Welfare, South Korea (02-1-2-0590).

PY - 2004/1/16

Y1 - 2004/1/16

N2 - Ligand activation of peroxisome proliferator-activated receptor γ (PPARγ) has been reported to induce growth inhibition and apoptosis in various cancers including hepatocellular carcinoma (HCC). However, the effect of hepatitis B virus X protein (HBx) on PPARγ activation has not been characterized in hepatitis B virus (HBV)-associated HCC. Herein, we demonstrated that HBx counteracted growth inhibition caused by PPARγ ligand in HBx-associated HCC cells. We found that HBx bound to DNA binding domain of PPARγ and HBx/PPARγ interaction blocked nuclear localization and binding to recognition site of PPARγ. HBx significantly suppressed a PPARγ-mediated transactivation. These results suggest that HBx modulates PPARγ function through protein-protein interaction.

AB - Ligand activation of peroxisome proliferator-activated receptor γ (PPARγ) has been reported to induce growth inhibition and apoptosis in various cancers including hepatocellular carcinoma (HCC). However, the effect of hepatitis B virus X protein (HBx) on PPARγ activation has not been characterized in hepatitis B virus (HBV)-associated HCC. Herein, we demonstrated that HBx counteracted growth inhibition caused by PPARγ ligand in HBx-associated HCC cells. We found that HBx bound to DNA binding domain of PPARγ and HBx/PPARγ interaction blocked nuclear localization and binding to recognition site of PPARγ. HBx significantly suppressed a PPARγ-mediated transactivation. These results suggest that HBx modulates PPARγ function through protein-protein interaction.

KW - Hepatitis B virus X protein

KW - Peroxisome proliferator-activated receptor γ

KW - Protein-protein interaction

KW - Transactivation

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DO - 10.1016/S0014-5793(03)01449-2

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AN - SCOPUS:9144250174

SN - 0014-5793

VL - 557

SP - 73

EP - 80

JO - FEBS Letters

JF - FEBS Letters

IS - 1-3

ER -

Hepatitis B virus X protein modulates peroxisome proliferator-activated receptor γ through protein-protein interaction

Abstract

Bibliographical note

Keywords

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