Heparin-immobilized biodegradable scaffolds for local and sustained release of angiogenic growth factor

Jun Jin Yoon, Hyun Jung Chung, Hyuk Jin Lee, Tae Gwan Park

Research output: Contribution to journalArticlepeer-review

125 Scopus citations

Abstract

Heparin-immobilized porous biodegradable scaffolds were fabricated to release basic fibroblast growth factor (bFGF) in a sustained manner. Heparin was covalently conjugated onto the surface of macroporous PLGA scaffolds fabricated by a gas-foaming/salt-leaching method. Sustained release of bFGF was successfully achieved for over 20 days due to high affinity of bFGF onto the immobilized heparin. It appears that bFGF release rate was regulated by the specific interaction between bFGF and heparin. The bFGF fraction released from the scaffolds maintained its bioactivity, as judged from determining the proliferation extent of human umbilical vein endothelial cells (HUVECs) in vitro. When heparin-immobilized scaffolds loaded with bFGF were implanted subcutaneously in vivo, they effectively induced the formation of blood vessels in the vicinity of the implant site. This study demonstrated that local and sustained delivery of angiogenic growth factor for tissue regeneration could be achieved by surface modification of porous scaffolds with heparin.

Original languageEnglish
Pages (from-to)934-942
Number of pages9
JournalJournal of Biomedical Materials Research - Part A
Volume79
Issue number4
DOIs
StatePublished - 15 Dec 2006

Keywords

  • Angiogenesis
  • Basic FGF
  • Heparin
  • Scaffolds
  • Sustained release

Fingerprint

Dive into the research topics of 'Heparin-immobilized biodegradable scaffolds for local and sustained release of angiogenic growth factor'. Together they form a unique fingerprint.

Cite this