Halobellus rufus sp. nov., an extremely halophilic archaeon isolated from non-purified solar salt

In Tae Cha, Kyung June Yim, Hye Seon Song, Hae Won Lee, Dong Wook Hyun, Kil Nam Kim, Myung Ji Seo, Daekyung Kim, Jong Soon Choi, Sung Jae Lee, Jin Woo Bae, Sung Keun Rhee, Hak Jong Choi, Jin Kyu Rhee, Young Do Nam, Seong Woon Roh

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A halophilic archaeon, designed strain CBA1103T, was isolated from non-purified solar salt. The cells of strain CBA1103T were observed to be Gram-stain negative and pleomorphic, and the colonies appear red. Strain CBA1103T was observed to grow between 20 and 55°C (optimum 37°C), and in NaCl concentrations of 10-30 % (w/v) (optimum 15 %) with 0-0.5 M MgSO4·7H2O (optimum 0.1 M) and at pH 6.0-9.0 (optimum pH 7.0). Additionally, the cells lyse in distilled water. The major polar lipids of strain CBA1103T are phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate and two glycolipids chromatographically identical to sulfated mannosyl glucosyl diether and manosyl glucosyl diether. Strain CBA1103T is shown to belong to the Halobellus genus and exhibits similarity to related taxa; the 16S rRNA gene sequence similarity between strain CBA1103T and Halobellus rarus 18362T, Hbs. limi 16811T, Hbs. litoreus JCM 17118T, Hbs. inordinatus YC20T, Hbs. clavatus TNN18 T and Hbs. salinus CSW2.24.4T is 97.3, 96.5, 96.5, 94.5, 94.5 and 93.7 %, respectively. The RNA polymerase subunit B gene sequence of strain CBA1103T shows 93.7 % similarity with the sequence of Hbs. litoreus JCM 17118T; the similarity is lower with sequences from the type strains of other species of Halobellus. The genomic DNA G+C content of strain CBA1103T was determined to be 67.0 mol% a value which is in the range of the genomic DNA G+C content of members of the genus Halobellus (61.5-69.2 mol%). These results suggest that strain CBA1103T should be considered to represent a new taxon for which the name Halobellus rufus sp. nov. is proposed, with the type strain CBA1103T (=CECT 8423 T =JCM 19434T).

Original languageEnglish
Pages (from-to)925-932
Number of pages8
JournalAntonie van Leeuwenhoek, International Journal of General and Molecular Microbiology
Issue number5
StatePublished - May 2014

Bibliographical note

Funding Information:
Acknowledgments This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science, and Technology (2012R1A1A2040922), and a project fund (C33730) to J. S. Choi from the Center for Analytical Research of Disaster Science at the Korea Basic Science Institute.


  • Haloarchaea
  • Halobellus rufus
  • Polyphasic taxonomy
  • Solar salt


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