Halobellus rufus sp. nov., an extremely halophilic archaeon isolated from non-purified solar salt

In Tae Cha; Kyung June Yim; Hye Seon Song; Hae Won Lee; Dong Wook Hyun; Kil Nam Kim; Myung Ji Seo; Daekyung Kim; Jong Soon Choi; Sung Jae Lee; Jin Woo Bae; Sung Keun Rhee; Hak Jong Choi; Jin Kyu Rhee; Young Do Nam; Seong Woon Roh

doi:10.1007/s10482-014-0147-y

Halobellus rufus sp. nov., an extremely halophilic archaeon isolated from non-purified solar salt

In Tae Cha, Kyung June Yim, Hye Seon Song, Hae Won Lee, Dong Wook Hyun, Kil Nam Kim, Myung Ji Seo, Daekyung Kim, Jong Soon Choi, Sung Jae Lee, Jin Woo Bae, Sung Keun Rhee, Hak Jong Choi, Jin Kyu Rhee, Young Do Nam, Seong Woon Roh

Department of Food Science & Biotechnology

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

A halophilic archaeon, designed strain CBA1103^T, was isolated from non-purified solar salt. The cells of strain CBA1103^T were observed to be Gram-stain negative and pleomorphic, and the colonies appear red. Strain CBA1103^T was observed to grow between 20 and 55°C (optimum 37°C), and in NaCl concentrations of 10-30 % (w/v) (optimum 15 %) with 0-0.5 M MgSO₄·7H₂O (optimum 0.1 M) and at pH 6.0-9.0 (optimum pH 7.0). Additionally, the cells lyse in distilled water. The major polar lipids of strain CBA1103^T are phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate and two glycolipids chromatographically identical to sulfated mannosyl glucosyl diether and manosyl glucosyl diether. Strain CBA1103^T is shown to belong to the Halobellus genus and exhibits similarity to related taxa; the 16S rRNA gene sequence similarity between strain CBA1103^T and Halobellus rarus 18362^T, Hbs. limi 16811^T, Hbs. litoreus JCM 17118^T, Hbs. inordinatus YC20^T, Hbs. clavatus TNN18 ^T and Hbs. salinus CSW2.24.4^T is 97.3, 96.5, 96.5, 94.5, 94.5 and 93.7 %, respectively. The RNA polymerase subunit B gene sequence of strain CBA1103^T shows 93.7 % similarity with the sequence of Hbs. litoreus JCM 17118^T; the similarity is lower with sequences from the type strains of other species of Halobellus. The genomic DNA G+C content of strain CBA1103^T was determined to be 67.0 mol% a value which is in the range of the genomic DNA G+C content of members of the genus Halobellus (61.5-69.2 mol%). These results suggest that strain CBA1103^T should be considered to represent a new taxon for which the name Halobellus rufus sp. nov. is proposed, with the type strain CBA1103^T (=CECT 8423 ^T =JCM 19434^T).

Original language	English
Pages (from-to)	925-932
Number of pages	8
Journal	Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology
Volume	105
Issue number	5
DOIs	https://doi.org/10.1007/s10482-014-0147-y
State	Published - May 2014

Bibliographical note

Funding Information:
Acknowledgments This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science, and Technology (2012R1A1A2040922), and a project fund (C33730) to J. S. Choi from the Center for Analytical Research of Disaster Science at the Korea Basic Science Institute.

Keywords

Haloarchaea
Halobellus rufus
Polyphasic taxonomy
Solar salt

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Cha, I. T., Yim, K. J., Song, H. S., Lee, H. W., Hyun, D. W., Kim, K. N., Seo, M. J., Kim, D., Choi, J. S., Lee, S. J., Bae, J. W., Rhee, S. K., Choi, H. J., Rhee, J. K., Nam, Y. D., & Roh, S. W. (2014). Halobellus rufus sp. nov., an extremely halophilic archaeon isolated from non-purified solar salt. Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology, 105(5), 925-932. https://doi.org/10.1007/s10482-014-0147-y

@article{58b6d35d3da646dcab7896eb8063d661,

title = "Halobellus rufus sp. nov., an extremely halophilic archaeon isolated from non-purified solar salt",

abstract = "A halophilic archaeon, designed strain CBA1103T, was isolated from non-purified solar salt. The cells of strain CBA1103T were observed to be Gram-stain negative and pleomorphic, and the colonies appear red. Strain CBA1103T was observed to grow between 20 and 55°C (optimum 37°C), and in NaCl concentrations of 10-30 % (w/v) (optimum 15 %) with 0-0.5 M MgSO4·7H2O (optimum 0.1 M) and at pH 6.0-9.0 (optimum pH 7.0). Additionally, the cells lyse in distilled water. The major polar lipids of strain CBA1103T are phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate and two glycolipids chromatographically identical to sulfated mannosyl glucosyl diether and manosyl glucosyl diether. Strain CBA1103T is shown to belong to the Halobellus genus and exhibits similarity to related taxa; the 16S rRNA gene sequence similarity between strain CBA1103T and Halobellus rarus 18362T, Hbs. limi 16811T, Hbs. litoreus JCM 17118T, Hbs. inordinatus YC20T, Hbs. clavatus TNN18 T and Hbs. salinus CSW2.24.4T is 97.3, 96.5, 96.5, 94.5, 94.5 and 93.7 %, respectively. The RNA polymerase subunit B gene sequence of strain CBA1103T shows 93.7 % similarity with the sequence of Hbs. litoreus JCM 17118T; the similarity is lower with sequences from the type strains of other species of Halobellus. The genomic DNA G+C content of strain CBA1103T was determined to be 67.0 mol% a value which is in the range of the genomic DNA G+C content of members of the genus Halobellus (61.5-69.2 mol%). These results suggest that strain CBA1103T should be considered to represent a new taxon for which the name Halobellus rufus sp. nov. is proposed, with the type strain CBA1103T (=CECT 8423 T =JCM 19434T).",

keywords = "Haloarchaea, Halobellus rufus, Polyphasic taxonomy, Solar salt",

author = "Cha, {In Tae} and Yim, {Kyung June} and Song, {Hye Seon} and Lee, {Hae Won} and Hyun, {Dong Wook} and Kim, {Kil Nam} and Seo, {Myung Ji} and Daekyung Kim and Choi, {Jong Soon} and Lee, {Sung Jae} and Bae, {Jin Woo} and Rhee, {Sung Keun} and Choi, {Hak Jong} and Rhee, {Jin Kyu} and Nam, {Young Do} and Roh, {Seong Woon}",

note = "Funding Information: Acknowledgments This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science, and Technology (2012R1A1A2040922), and a project fund (C33730) to J. S. Choi from the Center for Analytical Research of Disaster Science at the Korea Basic Science Institute.",

year = "2014",

month = may,

doi = "10.1007/s10482-014-0147-y",

language = "English",

volume = "105",

pages = "925--932",

journal = "Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology",

issn = "0003-6072",

publisher = "Springer Netherlands",

number = "5",

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Cha, IT, Yim, KJ, Song, HS, Lee, HW, Hyun, DW, Kim, KN, Seo, MJ, Kim, D, Choi, JS, Lee, SJ, Bae, JW, Rhee, SK, Choi, HJ, Rhee, JK, Nam, YD & Roh, SW 2014, 'Halobellus rufus sp. nov., an extremely halophilic archaeon isolated from non-purified solar salt', Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology, vol. 105, no. 5, pp. 925-932. https://doi.org/10.1007/s10482-014-0147-y

TY - JOUR

T1 - Halobellus rufus sp. nov., an extremely halophilic archaeon isolated from non-purified solar salt

AU - Cha, In Tae

AU - Yim, Kyung June

AU - Song, Hye Seon

AU - Lee, Hae Won

AU - Hyun, Dong Wook

AU - Kim, Kil Nam

AU - Seo, Myung Ji

AU - Kim, Daekyung

AU - Choi, Jong Soon

AU - Lee, Sung Jae

AU - Bae, Jin Woo

AU - Rhee, Sung Keun

AU - Choi, Hak Jong

AU - Rhee, Jin Kyu

AU - Nam, Young Do

AU - Roh, Seong Woon

N1 - Funding Information: Acknowledgments This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science, and Technology (2012R1A1A2040922), and a project fund (C33730) to J. S. Choi from the Center for Analytical Research of Disaster Science at the Korea Basic Science Institute.

PY - 2014/5

Y1 - 2014/5

N2 - A halophilic archaeon, designed strain CBA1103T, was isolated from non-purified solar salt. The cells of strain CBA1103T were observed to be Gram-stain negative and pleomorphic, and the colonies appear red. Strain CBA1103T was observed to grow between 20 and 55°C (optimum 37°C), and in NaCl concentrations of 10-30 % (w/v) (optimum 15 %) with 0-0.5 M MgSO4·7H2O (optimum 0.1 M) and at pH 6.0-9.0 (optimum pH 7.0). Additionally, the cells lyse in distilled water. The major polar lipids of strain CBA1103T are phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate and two glycolipids chromatographically identical to sulfated mannosyl glucosyl diether and manosyl glucosyl diether. Strain CBA1103T is shown to belong to the Halobellus genus and exhibits similarity to related taxa; the 16S rRNA gene sequence similarity between strain CBA1103T and Halobellus rarus 18362T, Hbs. limi 16811T, Hbs. litoreus JCM 17118T, Hbs. inordinatus YC20T, Hbs. clavatus TNN18 T and Hbs. salinus CSW2.24.4T is 97.3, 96.5, 96.5, 94.5, 94.5 and 93.7 %, respectively. The RNA polymerase subunit B gene sequence of strain CBA1103T shows 93.7 % similarity with the sequence of Hbs. litoreus JCM 17118T; the similarity is lower with sequences from the type strains of other species of Halobellus. The genomic DNA G+C content of strain CBA1103T was determined to be 67.0 mol% a value which is in the range of the genomic DNA G+C content of members of the genus Halobellus (61.5-69.2 mol%). These results suggest that strain CBA1103T should be considered to represent a new taxon for which the name Halobellus rufus sp. nov. is proposed, with the type strain CBA1103T (=CECT 8423 T =JCM 19434T).

AB - A halophilic archaeon, designed strain CBA1103T, was isolated from non-purified solar salt. The cells of strain CBA1103T were observed to be Gram-stain negative and pleomorphic, and the colonies appear red. Strain CBA1103T was observed to grow between 20 and 55°C (optimum 37°C), and in NaCl concentrations of 10-30 % (w/v) (optimum 15 %) with 0-0.5 M MgSO4·7H2O (optimum 0.1 M) and at pH 6.0-9.0 (optimum pH 7.0). Additionally, the cells lyse in distilled water. The major polar lipids of strain CBA1103T are phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate and two glycolipids chromatographically identical to sulfated mannosyl glucosyl diether and manosyl glucosyl diether. Strain CBA1103T is shown to belong to the Halobellus genus and exhibits similarity to related taxa; the 16S rRNA gene sequence similarity between strain CBA1103T and Halobellus rarus 18362T, Hbs. limi 16811T, Hbs. litoreus JCM 17118T, Hbs. inordinatus YC20T, Hbs. clavatus TNN18 T and Hbs. salinus CSW2.24.4T is 97.3, 96.5, 96.5, 94.5, 94.5 and 93.7 %, respectively. The RNA polymerase subunit B gene sequence of strain CBA1103T shows 93.7 % similarity with the sequence of Hbs. litoreus JCM 17118T; the similarity is lower with sequences from the type strains of other species of Halobellus. The genomic DNA G+C content of strain CBA1103T was determined to be 67.0 mol% a value which is in the range of the genomic DNA G+C content of members of the genus Halobellus (61.5-69.2 mol%). These results suggest that strain CBA1103T should be considered to represent a new taxon for which the name Halobellus rufus sp. nov. is proposed, with the type strain CBA1103T (=CECT 8423 T =JCM 19434T).

KW - Haloarchaea

KW - Halobellus rufus

KW - Polyphasic taxonomy

KW - Solar salt

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DO - 10.1007/s10482-014-0147-y

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JO - Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology

JF - Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology

IS - 5

ER -

Halobellus rufus sp. nov., an extremely halophilic archaeon isolated from non-purified solar salt

Abstract

Bibliographical note

Keywords

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