Halapricum salinum gen. nov., sp. nov., an extremely halophilic archaeon isolated from non-purified solar salt

Hye Seon Song, In Tae Cha, Kyung June Yim, Hae Won Lee, Dong Wook Hyun, Sung Jae Lee, Sung Keun Rhee, Kil Nam Kim, Daekyung Kim, Jong Soon Choi, Myung Ji Seo, Hak Jong Choi, Jin Woo Bae, Jin Kyu Rhee, Young Do Nam, Seong Woon Roh

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Abstract

A halophilic archaeal strain, designated CBA1105T, was isolated from non-purified solar salt. Strain CBA1105T was found to have three 16S rRNA genes, rrnA, rrnB and rrnC; similarities between the 16S rRNA gene sequences were 99.5-99.7 %. The phylogenetic analysis of the 16S rRNA gene sequences showed that strain CBA1105T forms a distinct clade with the strains of the closely related genera, Halorientalis and Halorhabdus, with similarities of 94.2 % and 93.9-94.2 %, respectively. Multilocus sequence analysis confirmed that strain CBA1105T is closely related to the genus Halorhabdus or Halorientalis. Growth of the strain was observed in 15-30 % NaCl (w/v; optimum 20 %), at 30-45°C (optimum 37°C) and pH 7.0-8.0 (optimum pH 7.0) and with 0-0.5 M MgCl2·6H2O (optimum 0.05-0.2 M). The cells of the strain were observed to be Gram-stain negative and pleomorphic with coccoid or ovoid-shape. The cells lysed in distilled water. Tweens 20, 40 and 80 were found to be hydrolysed but starch, casein and gelatine were not. The cells were unable to reduce nitrate under aerobic conditions. Assays for indole formation and urease activity were negative and no growth was observed under anaerobic conditions. Cells were found to be able to utilize l-glutamate, d-glucose, l-maltose, d-mannose and sucrose as sole carbon sources. The polar lipids were identified as phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, unidentified glycolipids and an unidentified phospholipid. The G+C content of strain CBA1105T was determined to be 66.0 mol%. The phenotypic, chemotaxonomic and phylogenetic properties suggest that the strain represents a novel species of a novel genus within the family Halobacteriaceae, for which the name Halapricum salinum is proposed with CBA1105T (= KCTC 4202 T = JCM 19729T) as the type strain.

Original languageEnglish
Pages (from-to)979-986
Number of pages8
JournalAntonie van Leeuwenhoek, International Journal of General and Molecular Microbiology
Volume105
Issue number5
DOIs
StatePublished - May 2014

Bibliographical note

Funding Information:
Acknowledgments This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) (2012R1A1A2040922) and a project fund (C33730) to J. S. Choi from the Center for Analytical Research of Disaster Science at the Korea Basic Science Institute.

Keywords

  • Halapricum salinum
  • Haloarchaeon
  • Polyphasic taxonomy
  • Solar salt

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