Growth inhibition and induction of G1 phase cell cycle arrest in human lung cancer cells by a phenylbutenoid dimer isolated from Zingiber cassumunar

Jong Won Lee, Hye Young Min, Ah Reum Han, Hwa Jin Chung, Eun Jung Park, Hyen Joo Park, Ji Young Hong, Eun Kyoung Seo, Sang Kook Lee

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

In our previous study, a novel phenylbutenoid dimer (±)-trans-3-(3, 4-dimethoxyphenyl)-4-[(E)-3,4-dimethoxystyryl] cyclohex-1-ene (PSC), isolated from Zingiber cassumunar ROXB. (Zingiberaceae), inhibited proliferation of various human cancer cells with the IC50 values ranging 10 to 30 μM. Prompted by these anti-proliferative effects, we performed additional studies in A549 human lung cancer cells in order to investigate the mechanism of action. PSC arrested cell cycle progression at the G0/G1 phase in a concentration- and time-dependent manner. PSC dose-dependently induced cyclin-dependent kinase (CDK) inhibitor p21 expression, whereas the expression of cyclin D1, cyclin A, CDK4, CDK2, and proliferating cell nuclear antigen (PCNA) were decreased by treatment with PSC. These results suggest that one of the anti-proliferative mechanisms of PSC is to suppress cell cycle progression by increasing p21 expression and down-regulating cyclins and CDKs. This study characterizes additional biological activity of this novel phenylbutenoid dimer and expands its therapeutic potential for cancer as a chemotherapeutic agent derived from natural products.

Original languageEnglish
Pages (from-to)1561-1564
Number of pages4
JournalBiological and Pharmaceutical Bulletin
Volume30
Issue number8
DOIs
StatePublished - Aug 2007

Keywords

  • A549 cell
  • G1 phase cell cycle arrest
  • Phenylbutenoid dimer
  • Zingiber cassumunar

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