Green Tea Extract Containing Piper retrofractum Fruit Ameliorates DSS-Induced Colitis via Modulating MicroRNA-21 Expression and NF-κB Activity

Mak Soon Lee, Jumi Lee, Yangha Kim

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7 Scopus citations

Abstract

The aim of the present study was to examine the effect of green tea extract containing Piper retrofractum fruit (GTP) on dextran-sulfate-sodium (DSS)-induced colitis, the regulatory mechanisms of microRNA (miR)-21, and the nuclear factor-κB (NF-κB) pathway. Different doses of GTP (50, 100, and 200 mg/kg) were administered orally once daily for 14 days, followed by GTP with 3% DSS for 7 days. Compared with the DSS-treated control, GTP administration alleviated clinical symptoms, including the disease activity index (DAI), colon shortening, and the degree of histological damage. Moreover, GTP suppressed miR-21 expression and NF-κB activity in colon tissue of DSS-induced colitis mice. The mRNA levels of inflammatory mediators, such as tumor necrosis factor-alpha (TNF-α), interleukin 6 (IL-6), interleukin-1β (IL-1β), inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2), were downregulated by GTP. Colonic nitric oxide (NO) and prostaglandin E2 (PGE2) production, and myeloperoxidase (MPO) activity were also lowered by GTP. Taken together, our results revealed that GTP inhibits DSS-induced colonic inflammation by suppressing miR-21 expression and NF-κB activity, suggesting that it may be used as a potential functional material for improving colitis.

Original languageEnglish
Article number2684
JournalNutrients
Volume14
Issue number13
DOIs
StatePublished - 1 Jul 2022

Bibliographical note

Funding Information:
Funding: This study was supported by the National Research Foundation of Korea (NRF), funded by the Korean Government (MSIT) (grant numbers 2012M3A9C4048761 and 2019R1A2C1002861);

Funding Information:
Funding: This study was supported by the National Research Foundation of Korea (NRF), funded by the Korean Government (MSIT) (grant numbers 2012M3A9C4048761 and 2019R1A2C1002861); and by Brain Korea 21 Four of the National Research Foundation funded by the Ministry of Education of Korea.

Publisher Copyright:
© 2022 by the authors. Licensee MDPI, Basel, Switzerland.

Keywords

  • NF-κB
  • Piper retrofractum
  • colitis
  • green tea
  • inflammatory response
  • microRNA

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