Green tea catechin enhances cholesterol 7α-hydroxylase gene expression in HepG2 cells

Mak Soon Lee, Ju Yeon Park, Hedley Freake, In Sook Kwun, Yangha Kim

Research output: Contribution to journalArticlepeer-review

36 Scopus citations

Abstract

Green tea catechins are known to have hypocholesterolaemic effects in animals and human subjects. In the present study, we investigated the effects of green tea catechins on the mRNA level and promoter activity of hepatic cholesterol 7α-hydroxylase (CYP7A1), the rate-limiting enzyme in the conversion of cholesterol to bile acids, in human hepatoma cells. Real-time PCR assays showed that different catechins, (-)-epicatechin gallate (ECG), (-)-epigallocatechin-3-gallate (EGCG), (-)-epigallocatechin (EGC) and (-)-epicatechin (EC), up regulated the CYP7A1 mRNA level by 5.5-, 4.2-, 2.9- and 1.9-fold, respectively, compared with the control. The -1312/+358 bp of the CYP7A1 promoter was subcloned into the pGL3 basic vector that includes luciferase as a reporter gene. ECG or EGCG significantly increased CYP7A1 promoter activity by 6.0- or 4.0-fold, respectively, compared with the control. Also, EGCG stimulated CYP7A1 at both mRNA level and promoter activity in a dose-dependent manner. These results suggest that the expression of the CYP7A1 gene may be directly regulated by green tea catechins at the transcriptional level.

Original languageEnglish
Pages (from-to)1182-1185
Number of pages4
JournalBritish Journal of Nutrition
Volume99
Issue number6
DOIs
StatePublished - 2008

Keywords

  • Cholesterol 7α-hydroxylase
  • CYP7A1
  • Green tea catechins
  • HepG2 cells
  • mRNA
  • Promoter activity

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