GMP and IMP are competitive inhibitors of CMY-10, an extended-spectrum class C β-lactamase

Jung Hyun Na, Young Jun An, Sun Shin Cha

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Nucleotides were effective in inhibiting the class C β-lactamase CMY-10. IMP was the most potent competitive inhibitor, with a Ki value of 16.2 μM. The crystal structure of CMY-10 complexed with GMP or IMP revealed that nucleotides fit into the R2 subsite of the active site with a unique vertical binding mode where the phosphate group at one terminus is deeply bound in the subsite and the base at the other terminus faces the solvent.

Original languageEnglish
Article numbere00098
JournalAntimicrobial Agents and Chemotherapy
Volume61
Issue number5
DOIs
StatePublished - May 2017

Keywords

  • Competitive inhibitor
  • GMP
  • IMP
  • β-lactamases

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