Glutathione reductase plays an anti-apoptotic role against oxidative stress in human hepatoma cells

Su Jung Kim; Hyun Joo Jung; Dong Hoon Hyun; Eun Hee Park; Young Myeong Kim; Chang Jin Lim

doi:10.1016/j.biochi.2010.03.007

Glutathione reductase plays an anti-apoptotic role against oxidative stress in human hepatoma cells

Su Jung Kim, Hyun Joo Jung, Dong Hoon Hyun, Eun Hee Park, Young Myeong Kim, Chang Jin Lim

Department of Life Science

Research output: Contribution to journal › Article › peer-review

43 Scopus citations

Abstract

The cellular roles of glutathione reductase (GR) in the reactive oxygen species (ROS)-induced apoptosis were studied using the HepG2 cells transfected with GR. The overexpression of GR caused a marked enhancement in reduced and oxidized glutathione (GSH/GSSG) ratio, and significantly decreased ROS levels in the stable transfectants. Hydrogen peroxide (H ₂O ₂), under the optimal condition for apoptosis, significantly decreased cellular viability and total GSH content, and rather increased ROS level, apoptotic percentage and caspase-3 activity in the mock-transfected cells. However, hydrogen peroxide could not largely generate these apoptotic changes in cellular viability, ROS level, apoptotic percentage, caspase-3 activity and total GSH content in the cells overexpressing GR. Taken together, GR may play a protective role against oxidative stress.

Original language	English
Pages (from-to)	927-932
Number of pages	6
Journal	Biochimie
Volume	92
Issue number	8
DOIs	https://doi.org/10.1016/j.biochi.2010.03.007
State	Published - Aug 2010

Bibliographical note

Funding Information:
This work was supported by the Korea Research Foundation Grant funded by the Korean Government (MOEHRD, Basic Research Promotion Fund) ( KRF-2007-313-C00465 ).

Keywords

Apoptosis
Glutathione
Glutathione reductase
HepG2
Hepatoma cells
Hydrogen peroxide
Reactive oxygen species

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10.1016/j.biochi.2010.03.007

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title = "Glutathione reductase plays an anti-apoptotic role against oxidative stress in human hepatoma cells",

abstract = "The cellular roles of glutathione reductase (GR) in the reactive oxygen species (ROS)-induced apoptosis were studied using the HepG2 cells transfected with GR. The overexpression of GR caused a marked enhancement in reduced and oxidized glutathione (GSH/GSSG) ratio, and significantly decreased ROS levels in the stable transfectants. Hydrogen peroxide (H 2O 2), under the optimal condition for apoptosis, significantly decreased cellular viability and total GSH content, and rather increased ROS level, apoptotic percentage and caspase-3 activity in the mock-transfected cells. However, hydrogen peroxide could not largely generate these apoptotic changes in cellular viability, ROS level, apoptotic percentage, caspase-3 activity and total GSH content in the cells overexpressing GR. Taken together, GR may play a protective role against oxidative stress.",

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author = "Kim, {Su Jung} and Jung, {Hyun Joo} and Hyun, {Dong Hoon} and Park, {Eun Hee} and Kim, {Young Myeong} and Lim, {Chang Jin}",

note = "Funding Information: This work was supported by the Korea Research Foundation Grant funded by the Korean Government (MOEHRD, Basic Research Promotion Fund) ( KRF-2007-313-C00465 ).",

year = "2010",

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doi = "10.1016/j.biochi.2010.03.007",

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AU - Kim, Su Jung

AU - Jung, Hyun Joo

AU - Hyun, Dong Hoon

AU - Park, Eun Hee

AU - Kim, Young Myeong

AU - Lim, Chang Jin

N1 - Funding Information: This work was supported by the Korea Research Foundation Grant funded by the Korean Government (MOEHRD, Basic Research Promotion Fund) ( KRF-2007-313-C00465 ).

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N2 - The cellular roles of glutathione reductase (GR) in the reactive oxygen species (ROS)-induced apoptosis were studied using the HepG2 cells transfected with GR. The overexpression of GR caused a marked enhancement in reduced and oxidized glutathione (GSH/GSSG) ratio, and significantly decreased ROS levels in the stable transfectants. Hydrogen peroxide (H 2O 2), under the optimal condition for apoptosis, significantly decreased cellular viability and total GSH content, and rather increased ROS level, apoptotic percentage and caspase-3 activity in the mock-transfected cells. However, hydrogen peroxide could not largely generate these apoptotic changes in cellular viability, ROS level, apoptotic percentage, caspase-3 activity and total GSH content in the cells overexpressing GR. Taken together, GR may play a protective role against oxidative stress.

AB - The cellular roles of glutathione reductase (GR) in the reactive oxygen species (ROS)-induced apoptosis were studied using the HepG2 cells transfected with GR. The overexpression of GR caused a marked enhancement in reduced and oxidized glutathione (GSH/GSSG) ratio, and significantly decreased ROS levels in the stable transfectants. Hydrogen peroxide (H 2O 2), under the optimal condition for apoptosis, significantly decreased cellular viability and total GSH content, and rather increased ROS level, apoptotic percentage and caspase-3 activity in the mock-transfected cells. However, hydrogen peroxide could not largely generate these apoptotic changes in cellular viability, ROS level, apoptotic percentage, caspase-3 activity and total GSH content in the cells overexpressing GR. Taken together, GR may play a protective role against oxidative stress.

KW - Apoptosis

KW - Glutathione

KW - Glutathione reductase

KW - HepG2

KW - Hepatoma cells

KW - Hydrogen peroxide

KW - Reactive oxygen species

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ER -

Glutathione reductase plays an anti-apoptotic role against oxidative stress in human hepatoma cells

Abstract

Bibliographical note

Keywords

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