Glucosamine exerts a neuroprotective effect via suppression of inflammation in rat brain ischemia/reperfusion injury

So Young Hwang, Joo Hyun Shin, Ji Sun Hwang, Song Yi Kim, Jin A. Shin, Eok Soo Oh, Seikwan Oh, Jung Bin Kim, Ja Kyung Lee, Inn Oc Han

Research output: Contribution to journalArticlepeer-review

106 Scopus citations


We investigated the neuroprotective effect of glucosamine (GlcN) in a rat middle cerebral artery occlusion model. At the highest dose used, intraperitoneal GlcN reduced infarct volume to 14.3% ± 7.4% that of untreated controls and afforded a reduction in motor impairment and neurological deficits. Neuroprotective effects were not reproduced by other amine sugars or acetylated-GlcN, and GlcN suppressed postischemic microglial activation. Moreover, GlcN suppressed lipopolysaccharide (LPS)-induced upregulation of proinflammatory mediators both in vivo and in culture systems using microglial or macrophage cells. The antiinflammatory effects of GlcN were mainly attributable to its ability to inhibit nuclear factor kappaB (NF-jB) activation. GlcN inhibited LPS-induced nuclear translocation and DNA binding of p65 to both NF-jB consensus sequence and NF-jB binding sequence of inducible nitric oxide synthase promoter. In addition, we found that GlcN strongly repressed p65 transactivation in BV2 cells using Gal4-p65 chimeras system. P65 displayed increased O-GlcNAcylation in response to LPS; this effect was also reversed by GlcN. The LPS-induced increase in p65 O-GlcNAcylation was paralleled by an increase in interaction with O-GlcNAc transferase, which was reversed by GlcN. Finally, our results suggest that GlcN or its derivatives may serve as novel neuroprotective or anti-inflammatory agents.

Original languageEnglish
Pages (from-to)1881-1892
Number of pages12
Issue number15
StatePublished - 15 Nov 2010


  • Glucosamine
  • NF-kappaB
  • O-GlcNAcylation


Dive into the research topics of 'Glucosamine exerts a neuroprotective effect via suppression of inflammation in rat brain ischemia/reperfusion injury'. Together they form a unique fingerprint.

Cite this