Ginseng saponin metabolite induces apoptosis in MCF-7 breast cancer cells through the modulation of AMP-activated protein kinase

Areum Daseul Kim; Kyoung Ah Kang; Rui Zhang; Chae Moon Lim; Hee Sun Kim; Dong Hyun Kim; You Jin Jeon; Chang Hyun Lee; Jinny Park; Weon Young Chang; Jin Won Hyun

doi:10.1016/j.etap.2010.04.008

Ginseng saponin metabolite induces apoptosis in MCF-7 breast cancer cells through the modulation of AMP-activated protein kinase

Areum Daseul Kim, Kyoung Ah Kang, Rui Zhang, Chae Moon Lim, Hee Sun Kim, Dong Hyun Kim, You Jin Jeon, Chang Hyun Lee, Jinny Park, Weon Young Chang, Jin Won Hyun

Department of Molecular Medicine

Research output: Contribution to journal › Article › peer-review

48 Scopus citations

Abstract

Previous studies have shown that the ginseng saponin metabolite, Compound K (20-O-d-glucopyranosyl-20(S)-protopanaxadiol, IH901), suppresses proliferation of various cancers and induces apoptosis. AMP-activated protein kinase (AMPK) is a sensor of cellular energy states and is involved in apoptosis of cancer cells. We hypothesized that Compound K may exert cytotoxicity in MCF-7 human breast cancer cells through modulation of AMPK, followed by a decrease in cyclooxygenase-2 (COX-2) expression. Compound K inhibited cell growth, induced apoptosis via generation of reactive oxygen species (ROS), as well as decreasing COX-2 expression and prostaglandin E₂ (PGE₂) levels. These effects of Compound K were induced via an AMPK-dependent pathway and were abrogated by a specific AMPK inhibitor. These results suggest that Compound K induced apoptosis by modulating AMPK-COX-2 signaling in MCF-7 human breast cancer cells.

Original language	English
Pages (from-to)	134-140
Number of pages	7
Journal	Environmental Toxicology and Pharmacology
Volume	30
Issue number	2
DOIs	https://doi.org/10.1016/j.etap.2010.04.008
State	Published - Sep 2010

Bibliographical note

Funding Information:
This work was supported by a research grant from Jeju National University Hospital.

Keywords

AMP-activated protein kinase
Apoptosis
Compound K
MCF-7
Reactive oxygen species

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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Cite this

Kim, A. D., Kang, K. A., Zhang, R., Lim, C. M., Kim, H. S., Kim, D. H., Jeon, Y. J., Lee, C. H., Park, J., Chang, W. Y., & Hyun, J. W. (2010). Ginseng saponin metabolite induces apoptosis in MCF-7 breast cancer cells through the modulation of AMP-activated protein kinase. Environmental Toxicology and Pharmacology, 30(2), 134-140. https://doi.org/10.1016/j.etap.2010.04.008

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title = "Ginseng saponin metabolite induces apoptosis in MCF-7 breast cancer cells through the modulation of AMP-activated protein kinase",

abstract = "Previous studies have shown that the ginseng saponin metabolite, Compound K (20-O-d-glucopyranosyl-20(S)-protopanaxadiol, IH901), suppresses proliferation of various cancers and induces apoptosis. AMP-activated protein kinase (AMPK) is a sensor of cellular energy states and is involved in apoptosis of cancer cells. We hypothesized that Compound K may exert cytotoxicity in MCF-7 human breast cancer cells through modulation of AMPK, followed by a decrease in cyclooxygenase-2 (COX-2) expression. Compound K inhibited cell growth, induced apoptosis via generation of reactive oxygen species (ROS), as well as decreasing COX-2 expression and prostaglandin E2 (PGE2) levels. These effects of Compound K were induced via an AMPK-dependent pathway and were abrogated by a specific AMPK inhibitor. These results suggest that Compound K induced apoptosis by modulating AMPK-COX-2 signaling in MCF-7 human breast cancer cells.",

keywords = "AMP-activated protein kinase, Apoptosis, Compound K, MCF-7, Reactive oxygen species",

author = "Kim, {Areum Daseul} and Kang, {Kyoung Ah} and Rui Zhang and Lim, {Chae Moon} and Kim, {Hee Sun} and Kim, {Dong Hyun} and Jeon, {You Jin} and Lee, {Chang Hyun} and Jinny Park and Chang, {Weon Young} and Hyun, {Jin Won}",

note = "Funding Information: This work was supported by a research grant from Jeju National University Hospital.",

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doi = "10.1016/j.etap.2010.04.008",

language = "English",

volume = "30",

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AU - Kim, Areum Daseul

AU - Kang, Kyoung Ah

AU - Zhang, Rui

AU - Lim, Chae Moon

AU - Kim, Hee Sun

AU - Kim, Dong Hyun

AU - Jeon, You Jin

AU - Lee, Chang Hyun

AU - Park, Jinny

AU - Chang, Weon Young

AU - Hyun, Jin Won

N1 - Funding Information: This work was supported by a research grant from Jeju National University Hospital.

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N2 - Previous studies have shown that the ginseng saponin metabolite, Compound K (20-O-d-glucopyranosyl-20(S)-protopanaxadiol, IH901), suppresses proliferation of various cancers and induces apoptosis. AMP-activated protein kinase (AMPK) is a sensor of cellular energy states and is involved in apoptosis of cancer cells. We hypothesized that Compound K may exert cytotoxicity in MCF-7 human breast cancer cells through modulation of AMPK, followed by a decrease in cyclooxygenase-2 (COX-2) expression. Compound K inhibited cell growth, induced apoptosis via generation of reactive oxygen species (ROS), as well as decreasing COX-2 expression and prostaglandin E2 (PGE2) levels. These effects of Compound K were induced via an AMPK-dependent pathway and were abrogated by a specific AMPK inhibitor. These results suggest that Compound K induced apoptosis by modulating AMPK-COX-2 signaling in MCF-7 human breast cancer cells.

AB - Previous studies have shown that the ginseng saponin metabolite, Compound K (20-O-d-glucopyranosyl-20(S)-protopanaxadiol, IH901), suppresses proliferation of various cancers and induces apoptosis. AMP-activated protein kinase (AMPK) is a sensor of cellular energy states and is involved in apoptosis of cancer cells. We hypothesized that Compound K may exert cytotoxicity in MCF-7 human breast cancer cells through modulation of AMPK, followed by a decrease in cyclooxygenase-2 (COX-2) expression. Compound K inhibited cell growth, induced apoptosis via generation of reactive oxygen species (ROS), as well as decreasing COX-2 expression and prostaglandin E2 (PGE2) levels. These effects of Compound K were induced via an AMPK-dependent pathway and were abrogated by a specific AMPK inhibitor. These results suggest that Compound K induced apoptosis by modulating AMPK-COX-2 signaling in MCF-7 human breast cancer cells.

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KW - Apoptosis

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KW - MCF-7

KW - Reactive oxygen species

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Ginseng saponin metabolite induces apoptosis in MCF-7 breast cancer cells through the modulation of AMP-activated protein kinase

Abstract

Bibliographical note

Keywords

UN SDGs

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