Germination and subsequent inactivation of bacillus subtilis spores by pulsed electric field treatment

J. K. Shin, S. J. Lee, H. Y. Cho, Y. R. Pyun, J. H. Lee, M. S. Chung

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18 Scopus citations

Abstract

The use of high-intensity pulsed electric field (PEF) as a nonthermal method for triggering spore germination was investigated. Spores suspended in 0.01% NaCl solution (ca. 108cells/mL) were treated with an exponential-decay pulse (20-50 kV/cm) at selected temperatures. The inactivation resulting from the direct effect of PEF treatment was less than 0.5 log cycles, even with a relatively high electric field intensity of 40 kV/cm. Most of the surviving PEF-treated spores germinated after inoculation into selected germination media (0.85% NaCl solution, nutrient broth [NB], or tryptic soy broth [TSB]) and subsequent incubation at 37C for 40 min. The extent of germination increased with the treatment temperature and electric field intensity. More than 98% of surviving spores in TSB germinated during incubation after PEF treatment with an electric field strength of 40 kV/cm for 1,000 μs at 50C. The rate of germination in nutrient-rich TSB medium was higher than that in NB.

Original languageEnglish
Pages (from-to)43-54
Number of pages12
JournalJournal of Food Processing and Preservation
Volume34
Issue number1
DOIs
StatePublished - Feb 2010

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