Gentiopicroside isolated from Gentiana scabra Bge. inhibits adipogenesis in 3T3-L1 cells and reduces body weight in diet-induced obese mice

Ra Yeong Choi, Sang Jip Nam, Hae In Lee, Jihye Lee, Alain S. Leutou, Ju Ri Ham, Mi Kyung Lee

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Gentiopicroside is a major active component of the Gentiana scabra Bge., which is commonly used as herbal medicine for the treatment of inflammation in Asia. Gentiopicroside significantly down-regulated expression of key adipogenic transcription factors (PPARγ, C/EBPα, SREBP-1c) and dose-dependently inhibited the lipid uptake-related gene (LPL), fatty acid transport-related gene (FABP4) and triglyceride (TG) synthesis-related gene (DGAT2), as well as fatty acid synthesis-related genes (FAS, SCD1), which resulted in reduced intracellular lipid droplet accumulation and TG content in 3T3-L1 cells. Gentiopicroside also down-regulated expression of inflammatory cytokine genes (NFκB1, TNFα, IL6) compared with vehicle. Oral administration of gentiopicroside (50 mg/kg) in mice fed with high-fat diet for 12 weeks resulted in reduced body weight and visceral fat mass compared with the control group. Overall, the results of this study showed that gentiopicroside had positive anti-obesity effects by regulating the expression of adipogenesis/lipogenesis-related genes and inflammatory genes in 3T3-L1, and that it effectively reduced body weight and visceral fat mass in vivo.

Original languageEnglish
Pages (from-to)1699-1704
Number of pages6
JournalBioorganic and Medicinal Chemistry Letters
Volume29
Issue number14
DOIs
StatePublished - 15 Jul 2019

Keywords

  • 3T3-L1
  • Adipogenesis
  • Gentiopicroside
  • High-fat diet
  • Inflammation
  • Obesity

Fingerprint

Dive into the research topics of 'Gentiopicroside isolated from Gentiana scabra Bge. inhibits adipogenesis in 3T3-L1 cells and reduces body weight in diet-induced obese mice'. Together they form a unique fingerprint.

Cite this