Genomic structure of the regulatory region of the voltage-gated calcium channel α1D

Hyung Lae Kim, Yoon Jeong Chang, Sang Moo Lee, Young Sook Hong

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


In excitable and endocrine organs, calcium influxes through the L-type voltage-gated calcium channel (VGCC) which is composed of four (α1, α2, β, and γ) subunits. Temporal and spatial expression of calcium channel activity is regulated by the transcription of α1 subunit. To elucidate the genomic organization of the VGCC α1D subunit gene, a genomic clone was isolated from the human genomic library and its sequence was analyzed. A 12 kb genomic clone contained the 5'-flanking regulatory region and first two exons was selected and the initiation site for α1D mRNA synthesis was examined by primer extension analysis. The major initiation site was found at the -523 NT position in the translation initiation site. The TATA box could not be found above the transcription initiation site. The CAT vector construct containing the 2.5 kb upstream region had high CAT activity on transfection to NG108-15 and PC12 cells, which confers the neuronal expression of the α1D gene.

Original languageEnglish
Pages (from-to)246-251
Number of pages6
JournalExperimental and Molecular Medicine
Issue number4
StatePublished - 31 Dec 1998


  • Calcium channel
  • Gene expression
  • Molecular cloning
  • Regulation


Dive into the research topics of 'Genomic structure of the regulatory region of the voltage-gated calcium channel α1D'. Together they form a unique fingerprint.

Cite this