Genetic landscape of open chromatin in yeast

The genetic basis of gene expression has been studied in various organisms [1]-[5]. For example, two different strains of Saccharomyces cerevisiae (BY and RM) were crossed to produce a number of different genetic recombinants, and their expression levels and genotypes were analyzed [1], [6]. We previously utilized this system to separate the cis-and transcomponents of variation in gene expression [7]. Tirosh et al. [8] profiled nucleosome patterns in the inter-specific hybrids of two yeast species to dissect cis-and trans-effects on nucleosome positioning. Recently, variations in the binding patterns of transcription factors (TFs) have begun to be studied [9]-[11].