Generation of a recombinant antibody for sensitive detection of Pseudomonas aeruginosa

Gyu Min Lim, Joo Kyung Kim, Eun Jung Kim, Chang Soo Lee, Wooseong Kim, Byung Gee Kim, Hee Jin Jeong

Research output: Contribution to journalArticlepeer-review

Abstract

Pseudomonas aeruginosa (P. aeruginosa) is a major pathogen that causes nosocomial infections and often exhibits antibiotic resistance. Therefore, the development of an accurate method for detecting P. aeruginosa is required to control P. aeruginosa-related outbreaks. In this study, we established an enzyme-linked immunosorbent assay method for the sensitive detection of three P. aeruginosa strains, UCBPP PA14, ATCC 27853, and multidrug-resistant ATCC BAA-2108. We produced a recombinant antibody (rAb) against P. aeruginosa V‐antigen (PcrV), which is a needle tip protein of the type III secretion system of P. aeruginosa using mammalian cells with high yield and purity, and confirmed its P. aeruginosa binding efficiency. The rAb was paired with commercial anti-P. aeruginosa Ab for a sandwich ELISA, resulting in an antigen-concentration-dependent response with a limit of detection value of 230 CFU/mL. These results suggest that the rAb produced herein can be used for the sensitive detection of P. aeruginosa with a wide range of applications in clinical diagnosis and point-of-care testing.

Original languageEnglish
Article number21
JournalBMC Biotechnology
Volume22
Issue number1
DOIs
StatePublished - Dec 2022

Keywords

  • Enzyme-linked immunosorbent assay
  • HEK293F cells
  • Point-of-care testing
  • Pseudomonas aeruginosa
  • Recombinant antibody

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