Gene expression regulation by agonist-independent constitutive signaling of Melanocortin-1 receptor

Ikjoo Seong, Jaesang Kim

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Background: Melanocortin-1 receptor (Mc1r), a key signaling receptor for melanogenesis, has been reported to mediate migration of B16F10 melanoma cells. Interestingly, this activity appears to be a part of the constitutive signaling of Mc1r. Methods: We carried out small interfering RNA-mediated knock-down of Mc1r on murine melanoma B16F10 cells and performed microarray analysis to characterize changes in the gene expression profile. Results: We isolated 22 and four genes whose expression decreased and increased, respectively, by 2.5-fold or higher as the result of Mc1r knock-down. Several down-regulated genes have been proposed to be involved in cell migration. Among these genes are several members of the chemokine gene family. Conclusion: We provide a gene set for further functional analyses of Mc1r. The Mc1r target genes we present may be particularly relevant for understanding the ligand-independent activity of Mc1r. Further examination of the mode of action may lead to novel strategies in regulating the migration and metastasis of melanoma cells.

Original languageEnglish
Pages (from-to)179-184
Number of pages6
JournalEndocrinology and Metabolism
Volume29
Issue number2
DOIs
StatePublished - 2014

Keywords

  • Chemokines
  • Melanocortin
  • Melanoma
  • Migration
  • Receptor
  • Type 1

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