Functional maturation of myeloid cells during in vitro differentiation from human cord blood CD34+ cells

Yu Jin Jung; So Youn Woo; Kyung Ha Ryu; Who Soon Chung; Jeong Hae Kie; Ju Young Seoh

Functional maturation of myeloid cells during in vitro differentiation from human cord blood CD34+ cells

Yu Jin Jung, So Youn Woo, Kyung Ha Ryu, Who Soon Chung, Jeong Hae Kie, Ju Young Seoh

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

CD34+ cells purified from human cord blood were expanded in the presence of several cytokines. The cultured cells were assayed for myeloid effector functions, including phagocytic activity, respiratory burst and microbicidal activity. The results showed that phagocytic activity was observed as early as day 6, irrespective of the type of cytokines used. By contrast, respiratory burst and microbicidal activity peaked on day 15 and were dependent on the type of cytokines used. In particular, granulocyte-colony stimulating factor and interleukin-6 markedly enhanced respiratory burst and microbicidal activity.

Original language	English
Pages (from-to)	1222-1223
Number of pages	2
Journal	Haematologica
Volume	87
Issue number	11
State	Published - 1 Nov 2002

Keywords

CD34 cells
Cord blood
Ex vivo expansion
Maturation
Myeloid

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title = "Functional maturation of myeloid cells during in vitro differentiation from human cord blood CD34+ cells",

abstract = "CD34+ cells purified from human cord blood were expanded in the presence of several cytokines. The cultured cells were assayed for myeloid effector functions, including phagocytic activity, respiratory burst and microbicidal activity. The results showed that phagocytic activity was observed as early as day 6, irrespective of the type of cytokines used. By contrast, respiratory burst and microbicidal activity peaked on day 15 and were dependent on the type of cytokines used. In particular, granulocyte-colony stimulating factor and interleukin-6 markedly enhanced respiratory burst and microbicidal activity.",

keywords = "CD34 cells, Cord blood, Ex vivo expansion, Maturation, Myeloid",

author = "Jung, {Yu Jin} and Woo, {So Youn} and Ryu, {Kyung Ha} and Chung, {Who Soon} and Kie, {Jeong Hae} and Seoh, {Ju Young}",

year = "2002",

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TY - JOUR

T1 - Functional maturation of myeloid cells during in vitro differentiation from human cord blood CD34+ cells

AU - Jung, Yu Jin

AU - Woo, So Youn

AU - Ryu, Kyung Ha

AU - Chung, Who Soon

AU - Kie, Jeong Hae

AU - Seoh, Ju Young

PY - 2002/11/1

Y1 - 2002/11/1

N2 - CD34+ cells purified from human cord blood were expanded in the presence of several cytokines. The cultured cells were assayed for myeloid effector functions, including phagocytic activity, respiratory burst and microbicidal activity. The results showed that phagocytic activity was observed as early as day 6, irrespective of the type of cytokines used. By contrast, respiratory burst and microbicidal activity peaked on day 15 and were dependent on the type of cytokines used. In particular, granulocyte-colony stimulating factor and interleukin-6 markedly enhanced respiratory burst and microbicidal activity.

AB - CD34+ cells purified from human cord blood were expanded in the presence of several cytokines. The cultured cells were assayed for myeloid effector functions, including phagocytic activity, respiratory burst and microbicidal activity. The results showed that phagocytic activity was observed as early as day 6, irrespective of the type of cytokines used. By contrast, respiratory burst and microbicidal activity peaked on day 15 and were dependent on the type of cytokines used. In particular, granulocyte-colony stimulating factor and interleukin-6 markedly enhanced respiratory burst and microbicidal activity.

KW - CD34 cells

KW - Cord blood

KW - Ex vivo expansion

KW - Maturation

KW - Myeloid

UR - http://www.scopus.com/inward/record.url?scp=0036855236&partnerID=8YFLogxK

M3 - Article

C2 - 12414353

AN - SCOPUS:0036855236

SN - 0390-6078

VL - 87

SP - 1222

EP - 1223

JO - Haematologica

JF - Haematologica

IS - 11

ER -

Functional maturation of myeloid cells during in vitro differentiation from human cord blood CD34+ cells

Abstract

Keywords

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