Fumonisin B1 induces apoptosis in sphingosine 1-phosphate lyase-null F9 cells through increase of sphingolipids levels

Seon Mi Pak, Nam Young Park, Myung Yong Park, Wan Jong Kim, Jong Hwa Lee, Seikwan Oh, Hwan Soo Yoo, Yong Moon Lee

Research output: Contribution to journalArticlepeer-review

Abstract

Apoptosis is essential for a variety of pathophysiological progress. Apoptosis induction by various agents changes cellular morphology, DNA content and lipid membrane composition. Recently, sphingosine 1-phosphate (S1P) is avidly released from not only platelets and erythrocytes but vascular endothelium. Here we established S1P releasing cells by deleting S1P lyase (F9-12 cells). We observed apoptosis induction by the treatment of fumonisin B1 (FB1) in F9-12 cells but not in F9 wild-type cells. We measured high amounts of accumulated S1P and dihydroS1P (DHS1P) in FB1-induced apoptotic F9-12 cells. We also showed DHS1P release in an early stage of the apoptosis induction by FB1 but not by phorbol 12-myristate 13-acetate (PMA)-induced apoptosis, suggesting differential apoptotic processes.

Original languageEnglish
Pages (from-to)95-99
Number of pages5
JournalBiomolecules and Therapeutics
Volume16
Issue number2
DOIs
StatePublished - Jun 2008

Keywords

  • Apoptosis
  • F9 cells
  • HPLC
  • Release
  • Sphingosine 1-phosphate

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