Fumonisin B1 induces apoptosis in sphingosine 1-phosphate lyase-null F9 cells through increase of sphingolipids levels

Seon Mi Pak; Nam Young Park; Myung Yong Park; Wan Jong Kim; Jong Hwa Lee; Seikwan Oh; Hwan Soo Yoo; Yong Moon Lee

doi:10.4062/biomolther.2008.16.2.095

Fumonisin B1 induces apoptosis in sphingosine 1-phosphate lyase-null F9 cells through increase of sphingolipids levels

Seon Mi Pak, Nam Young Park, Myung Yong Park, Wan Jong Kim, Jong Hwa Lee, Seikwan Oh, Hwan Soo Yoo, Yong Moon Lee

Department of Molecular Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

Apoptosis is essential for a variety of pathophysiological progress. Apoptosis induction by various agents changes cellular morphology, DNA content and lipid membrane composition. Recently, sphingosine 1-phosphate (S1P) is avidly released from not only platelets and erythrocytes but vascular endothelium. Here we established S1P releasing cells by deleting S1P lyase (F9-12 cells). We observed apoptosis induction by the treatment of fumonisin B1 (FB1) in F9-12 cells but not in F9 wild-type cells. We measured high amounts of accumulated S1P and dihydroS1P (DHS1P) in FB1-induced apoptotic F9-12 cells. We also showed DHS1P release in an early stage of the apoptosis induction by FB1 but not by phorbol 12-myristate 13-acetate (PMA)-induced apoptosis, suggesting differential apoptotic processes.

Original language	English
Pages (from-to)	95-99
Number of pages	5
Journal	Biomolecules and Therapeutics
Volume	16
Issue number	2
DOIs	https://doi.org/10.4062/biomolther.2008.16.2.095
State	Published - Jun 2008

Keywords

Apoptosis
F9 cells
HPLC
Release
Sphingosine 1-phosphate

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title = "Fumonisin B1 induces apoptosis in sphingosine 1-phosphate lyase-null F9 cells through increase of sphingolipids levels",

abstract = "Apoptosis is essential for a variety of pathophysiological progress. Apoptosis induction by various agents changes cellular morphology, DNA content and lipid membrane composition. Recently, sphingosine 1-phosphate (S1P) is avidly released from not only platelets and erythrocytes but vascular endothelium. Here we established S1P releasing cells by deleting S1P lyase (F9-12 cells). We observed apoptosis induction by the treatment of fumonisin B1 (FB1) in F9-12 cells but not in F9 wild-type cells. We measured high amounts of accumulated S1P and dihydroS1P (DHS1P) in FB1-induced apoptotic F9-12 cells. We also showed DHS1P release in an early stage of the apoptosis induction by FB1 but not by phorbol 12-myristate 13-acetate (PMA)-induced apoptosis, suggesting differential apoptotic processes.",

keywords = "Apoptosis, F9 cells, HPLC, Release, Sphingosine 1-phosphate",

author = "Pak, {Seon Mi} and Park, {Nam Young} and Park, {Myung Yong} and Kim, {Wan Jong} and Lee, {Jong Hwa} and Seikwan Oh and Yoo, {Hwan Soo} and Lee, {Yong Moon}",

year = "2008",

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doi = "10.4062/biomolther.2008.16.2.095",

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journal = "Biomolecules and Therapeutics",

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AU - Pak, Seon Mi

AU - Park, Nam Young

AU - Park, Myung Yong

AU - Kim, Wan Jong

AU - Lee, Jong Hwa

AU - Oh, Seikwan

AU - Yoo, Hwan Soo

AU - Lee, Yong Moon

PY - 2008/6

Y1 - 2008/6

N2 - Apoptosis is essential for a variety of pathophysiological progress. Apoptosis induction by various agents changes cellular morphology, DNA content and lipid membrane composition. Recently, sphingosine 1-phosphate (S1P) is avidly released from not only platelets and erythrocytes but vascular endothelium. Here we established S1P releasing cells by deleting S1P lyase (F9-12 cells). We observed apoptosis induction by the treatment of fumonisin B1 (FB1) in F9-12 cells but not in F9 wild-type cells. We measured high amounts of accumulated S1P and dihydroS1P (DHS1P) in FB1-induced apoptotic F9-12 cells. We also showed DHS1P release in an early stage of the apoptosis induction by FB1 but not by phorbol 12-myristate 13-acetate (PMA)-induced apoptosis, suggesting differential apoptotic processes.

AB - Apoptosis is essential for a variety of pathophysiological progress. Apoptosis induction by various agents changes cellular morphology, DNA content and lipid membrane composition. Recently, sphingosine 1-phosphate (S1P) is avidly released from not only platelets and erythrocytes but vascular endothelium. Here we established S1P releasing cells by deleting S1P lyase (F9-12 cells). We observed apoptosis induction by the treatment of fumonisin B1 (FB1) in F9-12 cells but not in F9 wild-type cells. We measured high amounts of accumulated S1P and dihydroS1P (DHS1P) in FB1-induced apoptotic F9-12 cells. We also showed DHS1P release in an early stage of the apoptosis induction by FB1 but not by phorbol 12-myristate 13-acetate (PMA)-induced apoptosis, suggesting differential apoptotic processes.

KW - Apoptosis

KW - F9 cells

KW - HPLC

KW - Release

KW - Sphingosine 1-phosphate

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SN - 1976-9148

VL - 16

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EP - 99

JO - Biomolecules and Therapeutics

JF - Biomolecules and Therapeutics

IS - 2

ER -

Fumonisin B1 induces apoptosis in sphingosine 1-phosphate lyase-null F9 cells through increase of sphingolipids levels

Abstract

Keywords

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