Fluorogenic probes for disease-relevant enzymes

Junji Zhang, Xianzhi Chai, Xiao Peng He, Hae Jo Kim, Juyoung Yoon, He Tian

Research output: Contribution to journalReview articlepeer-review

321 Scopus citations

Abstract

Traditional biochemical methods for enzyme detection are mainly based on antibody-based immunoassays, which lack the ability to monitor the spatiotemporal distribution and, in particular, the in situ activity of enzymes in live cells and in vivo. In this review, we comprehensively summarize recent progress that has been made in the development of small-molecule as well as material-based fluorogenic probes for sensitive detection of the activities of enzymes that are related to a number of human diseases. The principles utilized to design these probes as well as their applications are reviewed. Specific attention is given to fluorogenic probes that have been developed for analysis of the activities of enzymes including oxidases and reductases, those that act on biomacromolecules including DNAs, proteins/peptides/amino acids, carbohydrates and lipids, and those that are responsible for translational modifications. We envision that this review will serve as an ideal reference for practitioners as well as beginners in relevant research fields.

Original languageEnglish
Pages (from-to)683-722
Number of pages40
JournalChemical Society Reviews
Volume48
Issue number2
DOIs
StatePublished - 21 Jan 2019

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