Fluorescence resonance energy transfer based quantum dot-Aptasensor for the selective detection of microcystin-LR in eutrophic water

Eun Hee Lee, Ahjeong Son

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

A highly sensitive fluorescence resonance energy transfer (FRET)-based quantum dot (QD)-Aptasensor was developed for microcystin-LR (MC-LR) detection in eutrophic water. The FRET-based QD-Aptasensor was able to achieve an analytical limit of detection of 10−4 μg/L MC-LR with selectivity among different congeners (microcystin-YR, microcystin-LY, microcystin-LW, microcystin-RR, microcystin-LF, microcystin-LA, and Nodularin). The performance of QD-Aptasensor was demonstrated using both laboratory grown cyanobacterial culture (Microcystis aeruginosa) and environmental water samples. For the laboratory culture, the intracellular MC-LR concentrations changed according to the cyanobacterial growth curve. The QD-Aptasensor detected MC-LR up to 12.7–15.8 μg/L-cyanobacterial culture at early stationary phase. For the environmental samples, the MC-LR was measured at cell densities of 2.7 × 108 and 6.6 × 1010 cells/L-water, which correspond to 1.0 and 7.2 μg MC-LR/L-water, respectively. For comparison, the laboratory culture and environmental samples were analyzed by the conventional enzyme-linked immuno-sorbent assay (ELISA). The MC-LR concentrations of FRET-based QD-Aptasensor showed a strong positive correlation with those of ELISA (Pearson correlation coefficient, r = 0.981 and r = 0.862) for the laboratory culture and environmental samples, respectively.

Original languageEnglish
Pages (from-to)1493-1501
Number of pages9
JournalChemical Engineering Journal
Volume359
DOIs
StatePublished - 1 Mar 2019

Keywords

  • Aptamer
  • Cyanobacterial bloom
  • Cyanotoxin
  • Fluorescence resonance energy transfer (FRET)
  • Quantum dots (QDs)

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