TY - JOUR
T1 - Fluorescence resonance energy transfer based quantum dot-Aptasensor for the selective detection of microcystin-LR in eutrophic water
AU - Lee, Eun Hee
AU - Son, Ahjeong
N1 - Funding Information:
This work was supported by the National Research Foundation of Korea ( NRF-2017R1A2B4 005133 ).
Publisher Copyright:
© 2018 Elsevier B.V.
PY - 2019/3/1
Y1 - 2019/3/1
N2 - A highly sensitive fluorescence resonance energy transfer (FRET)-based quantum dot (QD)-Aptasensor was developed for microcystin-LR (MC-LR) detection in eutrophic water. The FRET-based QD-Aptasensor was able to achieve an analytical limit of detection of 10−4 μg/L MC-LR with selectivity among different congeners (microcystin-YR, microcystin-LY, microcystin-LW, microcystin-RR, microcystin-LF, microcystin-LA, and Nodularin). The performance of QD-Aptasensor was demonstrated using both laboratory grown cyanobacterial culture (Microcystis aeruginosa) and environmental water samples. For the laboratory culture, the intracellular MC-LR concentrations changed according to the cyanobacterial growth curve. The QD-Aptasensor detected MC-LR up to 12.7–15.8 μg/L-cyanobacterial culture at early stationary phase. For the environmental samples, the MC-LR was measured at cell densities of 2.7 × 108 and 6.6 × 1010 cells/L-water, which correspond to 1.0 and 7.2 μg MC-LR/L-water, respectively. For comparison, the laboratory culture and environmental samples were analyzed by the conventional enzyme-linked immuno-sorbent assay (ELISA). The MC-LR concentrations of FRET-based QD-Aptasensor showed a strong positive correlation with those of ELISA (Pearson correlation coefficient, r = 0.981 and r = 0.862) for the laboratory culture and environmental samples, respectively.
AB - A highly sensitive fluorescence resonance energy transfer (FRET)-based quantum dot (QD)-Aptasensor was developed for microcystin-LR (MC-LR) detection in eutrophic water. The FRET-based QD-Aptasensor was able to achieve an analytical limit of detection of 10−4 μg/L MC-LR with selectivity among different congeners (microcystin-YR, microcystin-LY, microcystin-LW, microcystin-RR, microcystin-LF, microcystin-LA, and Nodularin). The performance of QD-Aptasensor was demonstrated using both laboratory grown cyanobacterial culture (Microcystis aeruginosa) and environmental water samples. For the laboratory culture, the intracellular MC-LR concentrations changed according to the cyanobacterial growth curve. The QD-Aptasensor detected MC-LR up to 12.7–15.8 μg/L-cyanobacterial culture at early stationary phase. For the environmental samples, the MC-LR was measured at cell densities of 2.7 × 108 and 6.6 × 1010 cells/L-water, which correspond to 1.0 and 7.2 μg MC-LR/L-water, respectively. For comparison, the laboratory culture and environmental samples were analyzed by the conventional enzyme-linked immuno-sorbent assay (ELISA). The MC-LR concentrations of FRET-based QD-Aptasensor showed a strong positive correlation with those of ELISA (Pearson correlation coefficient, r = 0.981 and r = 0.862) for the laboratory culture and environmental samples, respectively.
KW - Aptamer
KW - Cyanobacterial bloom
KW - Cyanotoxin
KW - Fluorescence resonance energy transfer (FRET)
KW - Quantum dots (QDs)
UR - http://www.scopus.com/inward/record.url?scp=85056357980&partnerID=8YFLogxK
U2 - 10.1016/j.cej.2018.11.027
DO - 10.1016/j.cej.2018.11.027
M3 - Article
AN - SCOPUS:85056357980
SN - 1385-8947
VL - 359
SP - 1493
EP - 1501
JO - Chemical Engineering Journal
JF - Chemical Engineering Journal
ER -