Fluorescence Probe for Imaging N-Methyl- d -aspartate Receptors and Monitoring GSH Selectively Using Two-Photon Microscopy

Nahyun Kwon, Chang Su Lim, Gyeongju Ko, Jeongsun Ha, Dayoung Lee, Jun Yin, Hwan Myung Kim, Juyoung Yoon

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

N-Methyl-d-aspartate (NMDA) is an excitotoxic amino acid used to identify a specific subset of glutamate receptors. The activity of NMDA receptors is closely related to the redox level of the biological system. Glutathione (GSH) as an antioxidant plays a key role with regard to modulation of the redox environment. In this work we designed and developed a GSH-specific fluorescent probe with the capability of targeting NMDA receptors, which was composed of a two-photon naphthalimide fluorophore, a GSH-reactive group sulfonamide, and an ifenprodil targeting group for the NMDA receptor. This probe exhibited high selectivity toward GSH in comparison to other similar amino acids. Two-photon fluorescence microscopy allowed this probe to successfully monitor GSH in neuronal cells and hippocampal tissues with an excitation at 750 nm. It could serve as a potential practical imaging tool to explore the function of GSH and related biological processes in the brain.

Original languageEnglish
Pages (from-to)11612-11616
Number of pages5
JournalAnalytical Chemistry
Volume93
Issue number33
DOIs
StatePublished - 24 Aug 2021

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