Flow cytometry-based rapid detection of Staphylococcus aureus and Pseudomonas aeruginosa using fluorescent antibodies

Joo Kyung Kim; Hanool Yun; Chang Hun Yeom; Eun Jung Kim; Wooseong Kim; Chang Soo Lee; Byung Gee Kim; Hee Jin Jeong

doi:10.1039/d2ra05694a

Flow cytometry-based rapid detection of Staphylococcus aureus and Pseudomonas aeruginosa using fluorescent antibodies

Joo Kyung Kim
, Hanool Yun
, Chang Hun Yeom
, Eun Jung Kim
, Wooseong Kim
, Chang Soo Lee
, Byung Gee Kim
, Hee Jin Jeong

Department of Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Staphylococcus aureus (S. aureus) and Pseudomonas aeruginosa (P. aeruginosa) are major pathogens frequently detected in food and beverage poisoning, and persistent infections. Therefore, the development of a rapid method that can detect these pathogens before serious multiplication is required. In this study, we established a flow cytometry (FCM)-based detection method that allows rapid acquisition of cell populations in fluid samples by using a fluorescent antibody against S. aureus or P. aeruginosa. Using this method, we detected these pathogens with a 10³ to 10⁵ CFU order of limit of detection value within 1 hour. The FCM-based method for the detection of S. aureus and P. aeruginosa offers the possibility of high-throughput analysis of pathogens in food, environmental, and clinical sources.

Original language	English
Pages (from-to)	34660-34669
Number of pages	10
Journal	RSC Advances
Volume	12
Issue number	53
DOIs	https://doi.org/10.1039/d2ra05694a
State	Published - 2 Dec 2022

Bibliographical note

Funding Information:
This work was supported by the Korea Medical Device Development Fund grant funded by the Korea government (KMDF_PR_20200901_0073), NRF grants funded by the Korean government (2020R1I1A307411712, 2021H1D3A2A02096525), 2022 Hongik University Research Fund, and 2022 Hongik University Innovation Support Program Fund.

Publisher Copyright:
© 2022 The Royal Society of Chemistry.

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@article{71b78350df704f32a3f5fc91dd69caec,

title = "Flow cytometry-based rapid detection of Staphylococcus aureus and Pseudomonas aeruginosa using fluorescent antibodies",

abstract = "Staphylococcus aureus (S. aureus) and Pseudomonas aeruginosa (P. aeruginosa) are major pathogens frequently detected in food and beverage poisoning, and persistent infections. Therefore, the development of a rapid method that can detect these pathogens before serious multiplication is required. In this study, we established a flow cytometry (FCM)-based detection method that allows rapid acquisition of cell populations in fluid samples by using a fluorescent antibody against S. aureus or P. aeruginosa. Using this method, we detected these pathogens with a 103 to 105 CFU order of limit of detection value within 1 hour. The FCM-based method for the detection of S. aureus and P. aeruginosa offers the possibility of high-throughput analysis of pathogens in food, environmental, and clinical sources.",

author = "Kim, \{Joo Kyung\} and Hanool Yun and Yeom, \{Chang Hun\} and Kim, \{Eun Jung\} and Wooseong Kim and Lee, \{Chang Soo\} and Kim, \{Byung Gee\} and Jeong, \{Hee Jin\}",

note = "Funding Information: This work was supported by the Korea Medical Device Development Fund grant funded by the Korea government (KMDF\_PR\_20200901\_0073), NRF grants funded by the Korean government (2020R1I1A307411712, 2021H1D3A2A02096525), 2022 Hongik University Research Fund, and 2022 Hongik University Innovation Support Program Fund. Publisher Copyright: {\textcopyright} 2022 The Royal Society of Chemistry.",

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doi = "10.1039/d2ra05694a",

language = "English",

volume = "12",

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T1 - Flow cytometry-based rapid detection of Staphylococcus aureus and Pseudomonas aeruginosa using fluorescent antibodies

AU - Kim, Joo Kyung

AU - Yun, Hanool

AU - Yeom, Chang Hun

AU - Kim, Eun Jung

AU - Kim, Wooseong

AU - Lee, Chang Soo

AU - Kim, Byung Gee

AU - Jeong, Hee Jin

N1 - Funding Information: This work was supported by the Korea Medical Device Development Fund grant funded by the Korea government (KMDF_PR_20200901_0073), NRF grants funded by the Korean government (2020R1I1A307411712, 2021H1D3A2A02096525), 2022 Hongik University Research Fund, and 2022 Hongik University Innovation Support Program Fund. Publisher Copyright: © 2022 The Royal Society of Chemistry.

PY - 2022/12/2

Y1 - 2022/12/2

N2 - Staphylococcus aureus (S. aureus) and Pseudomonas aeruginosa (P. aeruginosa) are major pathogens frequently detected in food and beverage poisoning, and persistent infections. Therefore, the development of a rapid method that can detect these pathogens before serious multiplication is required. In this study, we established a flow cytometry (FCM)-based detection method that allows rapid acquisition of cell populations in fluid samples by using a fluorescent antibody against S. aureus or P. aeruginosa. Using this method, we detected these pathogens with a 103 to 105 CFU order of limit of detection value within 1 hour. The FCM-based method for the detection of S. aureus and P. aeruginosa offers the possibility of high-throughput analysis of pathogens in food, environmental, and clinical sources.

AB - Staphylococcus aureus (S. aureus) and Pseudomonas aeruginosa (P. aeruginosa) are major pathogens frequently detected in food and beverage poisoning, and persistent infections. Therefore, the development of a rapid method that can detect these pathogens before serious multiplication is required. In this study, we established a flow cytometry (FCM)-based detection method that allows rapid acquisition of cell populations in fluid samples by using a fluorescent antibody against S. aureus or P. aeruginosa. Using this method, we detected these pathogens with a 103 to 105 CFU order of limit of detection value within 1 hour. The FCM-based method for the detection of S. aureus and P. aeruginosa offers the possibility of high-throughput analysis of pathogens in food, environmental, and clinical sources.

UR - https://www.scopus.com/pages/publications/85144073974

U2 - 10.1039/d2ra05694a

DO - 10.1039/d2ra05694a

M3 - Article

AN - SCOPUS:85144073974

SN - 2046-2069

VL - 12

SP - 34660

EP - 34669

JO - RSC Advances

JF - RSC Advances

IS - 53

ER -

Flow cytometry-based rapid detection of Staphylococcus aureus and Pseudomonas aeruginosa using fluorescent antibodies

Abstract

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