Flow cytometry-based rapid detection of Staphylococcus aureus and Pseudomonas aeruginosa using fluorescent antibodies

Joo Kyung Kim; Hanool Yun; Chang Hun Yeom; Eun Jung Kim; Wooseong Kim; Chang Soo Lee; Byung Gee Kim; Hee Jin Jeong

doi:10.1039/d2ra05694a

Flow cytometry-based rapid detection of Staphylococcus aureus and Pseudomonas aeruginosa using fluorescent antibodies

Joo Kyung Kim, Hanool Yun, Chang Hun Yeom, Eun Jung Kim, Wooseong Kim, Chang Soo Lee, Byung Gee Kim, Hee Jin Jeong

Department of Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Staphylococcus aureus (S. aureus) and Pseudomonas aeruginosa (P. aeruginosa) are major pathogens frequently detected in food and beverage poisoning, and persistent infections. Therefore, the development of a rapid method that can detect these pathogens before serious multiplication is required. In this study, we established a flow cytometry (FCM)-based detection method that allows rapid acquisition of cell populations in fluid samples by using a fluorescent antibody against S. aureus or P. aeruginosa. Using this method, we detected these pathogens with a 10³ to 10⁵ CFU order of limit of detection value within 1 hour. The FCM-based method for the detection of S. aureus and P. aeruginosa offers the possibility of high-throughput analysis of pathogens in food, environmental, and clinical sources.

Original language	English
Pages (from-to)	34660-34669
Number of pages	10
Journal	RSC Advances
Volume	12
Issue number	53
DOIs	https://doi.org/10.1039/d2ra05694a
State	Published - 2 Dec 2022

Bibliographical note

Funding Information:
This work was supported by the Korea Medical Device Development Fund grant funded by the Korea government (KMDF_PR_20200901_0073), NRF grants funded by the Korean government (2020R1I1A307411712, 2021H1D3A2A02096525), 2022 Hongik University Research Fund, and 2022 Hongik University Innovation Support Program Fund.

Publisher Copyright:
© 2022 The Royal Society of Chemistry.

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title = "Flow cytometry-based rapid detection of Staphylococcus aureus and Pseudomonas aeruginosa using fluorescent antibodies",

abstract = "Staphylococcus aureus (S. aureus) and Pseudomonas aeruginosa (P. aeruginosa) are major pathogens frequently detected in food and beverage poisoning, and persistent infections. Therefore, the development of a rapid method that can detect these pathogens before serious multiplication is required. In this study, we established a flow cytometry (FCM)-based detection method that allows rapid acquisition of cell populations in fluid samples by using a fluorescent antibody against S. aureus or P. aeruginosa. Using this method, we detected these pathogens with a 103 to 105 CFU order of limit of detection value within 1 hour. The FCM-based method for the detection of S. aureus and P. aeruginosa offers the possibility of high-throughput analysis of pathogens in food, environmental, and clinical sources.",

author = "Kim, {Joo Kyung} and Hanool Yun and Yeom, {Chang Hun} and Kim, {Eun Jung} and Wooseong Kim and Lee, {Chang Soo} and Kim, {Byung Gee} and Jeong, {Hee Jin}",

note = "Funding Information: This work was supported by the Korea Medical Device Development Fund grant funded by the Korea government (KMDF_PR_20200901_0073), NRF grants funded by the Korean government (2020R1I1A307411712, 2021H1D3A2A02096525), 2022 Hongik University Research Fund, and 2022 Hongik University Innovation Support Program Fund. Publisher Copyright: {\textcopyright} 2022 The Royal Society of Chemistry.",

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AU - Kim, Joo Kyung

AU - Yun, Hanool

AU - Yeom, Chang Hun

AU - Kim, Eun Jung

AU - Kim, Wooseong

AU - Lee, Chang Soo

AU - Kim, Byung Gee

AU - Jeong, Hee Jin

N1 - Funding Information: This work was supported by the Korea Medical Device Development Fund grant funded by the Korea government (KMDF_PR_20200901_0073), NRF grants funded by the Korean government (2020R1I1A307411712, 2021H1D3A2A02096525), 2022 Hongik University Research Fund, and 2022 Hongik University Innovation Support Program Fund. Publisher Copyright: © 2022 The Royal Society of Chemistry.

PY - 2022/12/2

Y1 - 2022/12/2

N2 - Staphylococcus aureus (S. aureus) and Pseudomonas aeruginosa (P. aeruginosa) are major pathogens frequently detected in food and beverage poisoning, and persistent infections. Therefore, the development of a rapid method that can detect these pathogens before serious multiplication is required. In this study, we established a flow cytometry (FCM)-based detection method that allows rapid acquisition of cell populations in fluid samples by using a fluorescent antibody against S. aureus or P. aeruginosa. Using this method, we detected these pathogens with a 103 to 105 CFU order of limit of detection value within 1 hour. The FCM-based method for the detection of S. aureus and P. aeruginosa offers the possibility of high-throughput analysis of pathogens in food, environmental, and clinical sources.

AB - Staphylococcus aureus (S. aureus) and Pseudomonas aeruginosa (P. aeruginosa) are major pathogens frequently detected in food and beverage poisoning, and persistent infections. Therefore, the development of a rapid method that can detect these pathogens before serious multiplication is required. In this study, we established a flow cytometry (FCM)-based detection method that allows rapid acquisition of cell populations in fluid samples by using a fluorescent antibody against S. aureus or P. aeruginosa. Using this method, we detected these pathogens with a 103 to 105 CFU order of limit of detection value within 1 hour. The FCM-based method for the detection of S. aureus and P. aeruginosa offers the possibility of high-throughput analysis of pathogens in food, environmental, and clinical sources.

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VL - 12

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EP - 34669

JO - RSC Advances

JF - RSC Advances

IS - 53

ER -

Flow cytometry-based rapid detection of Staphylococcus aureus and Pseudomonas aeruginosa using fluorescent antibodies

Abstract

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