Abstract
High-throughput screening of cells expressing active catalytic antibody clones by flow cytometry is described. A fluorogenic retro-aldol retro-Michael substrate was designed and synthesized with incorporation of a chloromethyl moiety for intracellular retention. Hybridoma or transfected mammalian cells expressing catalytic antibody molecules could be rapidly isolated.
Original language | English |
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Pages (from-to) | 4065-4068 |
Number of pages | 4 |
Journal | Bioorganic and Medicinal Chemistry Letters |
Volume | 14 |
Issue number | 15 |
DOIs | |
State | Published - 2 Aug 2004 |
Bibliographical note
Funding Information:This study was supported in part by the NIH (CA27489) and The Skaggs Institute for Chemical Biology.
Keywords
- Aldol
- Aldolase
- Catalytic antibody
- Fluorescence-based screening