Flow cytometric screening of aldolase catalytic antibodies

Hyunbo Shim, Amelie Karlström, Sofia M. Touami, Roberta P. Fuller, Carlos F. Barbas

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

High-throughput screening of cells expressing active catalytic antibody clones by flow cytometry is described. A fluorogenic retro-aldol retro-Michael substrate was designed and synthesized with incorporation of a chloromethyl moiety for intracellular retention. Hybridoma or transfected mammalian cells expressing catalytic antibody molecules could be rapidly isolated.

Original languageEnglish
Pages (from-to)4065-4068
Number of pages4
JournalBioorganic and Medicinal Chemistry Letters
Volume14
Issue number15
DOIs
StatePublished - 2 Aug 2004

Keywords

  • Aldol
  • Aldolase
  • Catalytic antibody
  • Fluorescence-based screening

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