TY - GEN
T1 - Finger-powered bead-in-droplet microfluidic system for point-of-care diagnostics
AU - Iwai, K.
AU - Sochol, R. D.
AU - Lee, L. P.
AU - Lin, L.
PY - 2012
Y1 - 2012
N2 - In this paper, we present a versatile 'human-powered' microfluidic system that encapsulates microbeads within droplets to perform a medical diagnostics assay. Point-of-care (POC) microfluidic devices hold great promise for medicinal applications throughout the world. In particular, portable, low-cost systems that can be operated by non-medical personnel without electrical supplies are desired. Here we present a PDMS microfluidic device (2.8 cm x 1.9 cm x 0.8 cm) to achieve four distinct accomplishments: (i) pressing force from a single human finger simultaneously actuates the flow for three distinct solutions/ suspensions in parallel, (ii) functionalized "detection" microbeads (φ = 15 μ m) and biological reagents are simultaneously encapsulated together within microdroplets (φ ≈ 50 μm), and (iii) novel trapping architectures are utilized to ultimately immobilize the microbead-containing microdroplets for fluorescence detection. The presented system was employed to detect the inflammatory cytokine, interferon-gamma (IFN-γ), via aptamer beacons conjugated to microbeads - which represents the first time IFN-γ detection has been achieved using microbeads inside microdroplets.
AB - In this paper, we present a versatile 'human-powered' microfluidic system that encapsulates microbeads within droplets to perform a medical diagnostics assay. Point-of-care (POC) microfluidic devices hold great promise for medicinal applications throughout the world. In particular, portable, low-cost systems that can be operated by non-medical personnel without electrical supplies are desired. Here we present a PDMS microfluidic device (2.8 cm x 1.9 cm x 0.8 cm) to achieve four distinct accomplishments: (i) pressing force from a single human finger simultaneously actuates the flow for three distinct solutions/ suspensions in parallel, (ii) functionalized "detection" microbeads (φ = 15 μ m) and biological reagents are simultaneously encapsulated together within microdroplets (φ ≈ 50 μm), and (iii) novel trapping architectures are utilized to ultimately immobilize the microbead-containing microdroplets for fluorescence detection. The presented system was employed to detect the inflammatory cytokine, interferon-gamma (IFN-γ), via aptamer beacons conjugated to microbeads - which represents the first time IFN-γ detection has been achieved using microbeads inside microdroplets.
UR - http://www.scopus.com/inward/record.url?scp=84860491102&partnerID=8YFLogxK
U2 - 10.1109/MEMSYS.2012.6170343
DO - 10.1109/MEMSYS.2012.6170343
M3 - Conference contribution
AN - SCOPUS:84860491102
SN - 9781467303248
T3 - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
SP - 949
EP - 952
BT - 2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012
T2 - 2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012
Y2 - 29 January 2012 through 2 February 2012
ER -