Filaggrin knockdown and Toll-like receptor 3 (TLR3) stimulation enhanced the production of thymic stromal lymphopoietin (TSLP) from epidermal layers

Kyung Ho Lee, Kyung Ah Cho, Ji Yoon Kim, Jin Young Kim, Ji Hae Baek, So Youn Woo, Jin Woo Kim

Research output: Contribution to journalLetterpeer-review

63 Scopus citations

Abstract

Keratinocytes constitute the first-line barrier against exogenous antigens and contain Toll-like receptors (TLRs), which function as pattern-recognition molecules to activate antimicrobial innate immune responses. In an effort to ascertain whether or not filaggrin (filament-aggregating protein) expression affected the TLR-mediated responses of keratinocytes, we transfected filaggrin siRNA into HaCaT human keratinocyte cells and determined that thymic stromal lymphopoietin (TSLP) and IL-6 secretion were increased by poly(I:C) stimulus. Additionally, TSLP expression is increased in filaggrin knockdown as well as TLR3 stimulation in reconstituted human epidermal layers. Therefore, the findings of this study show that reduced filaggrin levels may influence innate immune responses via TLR stimuli and may contribute to the pathogenesis of inflammatory skin disease via TSLP expression.

Original languageEnglish
Pages (from-to)149-151
Number of pages3
JournalExperimental Dermatology
Volume20
Issue number2
DOIs
StatePublished - Feb 2011

Keywords

  • Filaggrin
  • Keratinocytes
  • Thymic stromal lymphopoietin
  • Toll-like receptor

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