Fijiolides A and B, inhibitors of TNF-α-induced NFκB activation, from a marine-derived sediment bacterium of the genus Nocardiopsis

Sang Jip Nam, Susana P. Gaudêncio, Christopher A. Kauffman, Paul R. Jensen, Tamara P. Kondratyuk, Laura E. Marler, John M. Pezzuto, William Fenical

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Abstract

Fijiolide A, a potent inhibitor of TNF-α-induced NFκB activation, along with fijiolide B, were isolated from a marine-derived bacterium of the genus Nocardiopsis. The planar structures of fijiolides A (1) and B (2) were elucidated by interpretation of 2D NMR spectroscopic data, while the absolute configurations of these compounds were defined by interpretation of circular dichroism and 2D NMR data combined with application of the advanced Mosher's method. Fijiolides A and B are related to several recently isolated chloroaromatic compounds, which appear to be the Bergman cyclization products of enediyne precursors. Fijiolide A reduced TNF-α-induced NFκB activation by 70.3%, with an IC50 value of 0.57 μM. Fijiolide B demonstrated less inhibition, only 46.5%, without dose dependence. The same pattern was also observed with quinone reductase (QR) activity: fijiolide A was found to induce quinone reductase-1 (QR1) with an induction ratio of 3.5 at a concentration of 20 μg/mL (28.4 μM). The concentration required to double the activity was 1.8 μM. Fijiolide B did not affect QR1 activity, indicating the importance of the nitrogen substitution pattern for biological activity. On the basis of these data, fijiolide A is viewed as a promising lead for more advanced anticancer testing.

Original languageEnglish
Pages (from-to)1080-1086
Number of pages7
JournalJournal of Natural Products
Volume73
Issue number6
DOIs
StatePublished - 25 Jun 2010

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