Fermentative hydrogen production by the newly isolated Enterobacter asburiae SNU-1

Jong Hwan Shin, Jong Hyun Yoon, Kyoung Ahn Eun Kyoung Ahn, Mi Sun Kim, Sang Jun Sim, Tai Hyun Park

Research output: Contribution to journalArticlepeer-review

91 Scopus citations


A new fermentative hydrogen-producing bacterium was isolated from a domestic landfill and identified as Enterobacter asburiae using 16S rRNA gene sequencing and DNA-DNA hybridization methods. The isolated bacterium, designated as Enterobacter asburiae SNU-1, is a new species that has never been examined as a potential hydrogen-producing bacterium. This study examined the hydrogen-producing ability of Enterobacter asburiae SNU-1. During fermentation, the hydrogen was mainly produced in the stationary phase. The hydrogen yield based on the formate consumption was 0.43 mol hydrogen/mol formate. This strain was able to produce hydrogen over a wide range of pH (4-7.5), with the optimum pH being pH 7. The level of hydrogen production was also affected by the initial glucose concentration, and the optimum value was found to be 25 g glucose/l. The maximum and overall hydrogen productivities were 398 and 174 ml/l/hr, respectively, at pH 7 with an initial glucose concentration of 25 g/l. This strain could produce hydrogen from glucose and many other carbon sources such as fructose, sucrose, and sorbitol.

Original languageEnglish
Pages (from-to)192-199
Number of pages8
JournalInternational Journal of Hydrogen Energy
Issue number2
StatePublished - Feb 2007

Bibliographical note

Funding Information:
This research was performed for the Hydrogen Energy R&D Center, one of the 21st Century Frontier R&D Programs, funded by the Ministry of Science and Technology of Korea.


  • Enterobacter asburiae SNU-1
  • Fermentation
  • Formate decomposition
  • Hydrogen production


Dive into the research topics of 'Fermentative hydrogen production by the newly isolated Enterobacter asburiae SNU-1'. Together they form a unique fingerprint.

Cite this