Fed-batch culture of insect cells with exponential feeding of amino acid and yeastolate solution

J. H. Kim, E. J. Kim, T. H. Park

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


Amino acids rather than sugars are the primary limiting substrates for the culture of insect cells in a Grace's medium. When cultures are supplemented with amino acids, the yeastolate components other than the amino acids become the secondary limiting substrates. For the fed-batch culture of insect cells, a solution containing concentrated amino acids and yeastolate was supplied using an exponential feed flow rate calculated from mass balance equations. During the batch period the specific growth rate was 0.02 h-1, whereas during the fed-batch period it was measured as 0.007 and 0.012 h-1 on the basis of the cell numbers and the dry cell weight, respectively. This difference in the specific growth rates in the fed-batch period is caused by an increase in the cell size during this period. Furthermore, in fed-batch cultures, dissolved oxygen was found to be a limiting factor for high cell-density cultures.

Original languageEnglish
Pages (from-to)367-370
Number of pages4
JournalBioprocess Engineering
Issue number4
StatePublished - 2000

Bibliographical note

Funding Information:
Β. H. Γim Department of Genetic ∆ngineering, Sung Γyun Γwan Εniversity, Chunchun-Dong 300, Suwon 440-746, Γorea ∆. Β. Γim, T. H. Park (&) School of Chemical ∆ngineering, Seoul National Εniversity, Γwanak-Gu Shilim-Dong San 56-1, Seoul 151-742, Γorea The authors wish to acknowledge the financial support of the Γorea Science Ζ ∆ngineering Foundation through Nano Bio-∆lectronic Ζ System Center. This work was supported in part by the Brain Γorea21 Program from the Ministry of ∆ducation


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