Abstract
Amino acids rather than sugars are the primary limiting substrates for the culture of insect cells in a Grace's medium. When cultures are supplemented with amino acids, the yeastolate components other than the amino acids become the secondary limiting substrates. For the fed-batch culture of insect cells, a solution containing concentrated amino acids and yeastolate was supplied using an exponential feed flow rate calculated from mass balance equations. During the batch period the specific growth rate was 0.02 h-1, whereas during the fed-batch period it was measured as 0.007 and 0.012 h-1 on the basis of the cell numbers and the dry cell weight, respectively. This difference in the specific growth rates in the fed-batch period is caused by an increase in the cell size during this period. Furthermore, in fed-batch cultures, dissolved oxygen was found to be a limiting factor for high cell-density cultures.
| Original language | English |
|---|---|
| Pages (from-to) | 367-370 |
| Number of pages | 4 |
| Journal | Bioprocess Engineering |
| Volume | 23 |
| Issue number | 4 |
| DOIs | |
| State | Published - 2000 |
Bibliographical note
Funding Information:Β. H. Γim Department of Genetic ∆ngineering, Sung Γyun Γwan Εniversity, Chunchun-Dong 300, Suwon 440-746, Γorea ∆. Β. Γim, T. H. Park (&) School of Chemical ∆ngineering, Seoul National Εniversity, Γwanak-Gu Shilim-Dong San 56-1, Seoul 151-742, Γorea The authors wish to acknowledge the financial support of the Γorea Science Ζ ∆ngineering Foundation through Nano Bio-∆lectronic Ζ System Center. This work was supported in part by the Brain Γorea21 Program from the Ministry of ∆ducation