Fatty acid hydration activity of a recombinant Escherichia coli-based biocatalyst is improved through targeting the oleate hydratase into the periplasm

Sang Min Jung, Joo Hyun Seo, Jung Hoo Lee, Jin Byung Park, Jin Ho Seo

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Whole-cell biotransformation of fatty acids can be influenced by the activities of catalytic enzymes and by the efficiency of substrate transport into host cells. Here, we improved fatty acid hydration activity of the recombinant Escherichia coli expressing an oleate hydratase of Stenotrophomonas maltophilia by targeting the catalytic enzyme into the periplasm instead of the cytoplasm. Recombinant E. coli producing OhyA in the periplasm under guidance of the PelB signal sequence (E. coli OhyA_PP) exhibited significantly greater hydration activity with oleic acid and linoleic acid compared to a recombinant E. coli producing OhyA in the cytoplasm (E. coli OhyA_CS). For example, the oleate double bond hydration rate of E. coli OhyA_PP was >400 μmol/g dry cells/min (400 U/g dry cells), which is >10-fold higher than that of E. coli OhyA_CS. As the specific activities of the enzymes targeted into the cytoplasm and periplasm were comparable, we assumed that targeting OhyA into the periplasm could accelerate fatty acid transport to the catalytic enzymes by skipping the major mass transport barrier of the cytoplasmic membrane. Our results will contribute to the development of whole-cell biocatalysts for fatty acid biotransformation. Whole-cell biotransformation of fatty acids can be influenced by the activities of catalytic enzymes as well as the efficiency of substrate transport into host cells. In this work, the authors improve fatty acid hydration activity of the recombinant Escherichia coli which expresses an oleate hydratase of Stenotrophomonas maltophilia by increasing the efficiency of substrate transport into catalytic enzymes. This work will contribute to the development of whole-cell biocatalysts for fatty acid biotransformation.

Original languageEnglish
Pages (from-to)1887-1893
Number of pages7
JournalBiotechnology Journal
Volume10
Issue number12
DOIs
StatePublished - 1 Dec 2015

Keywords

  • FAD-dependent oleate hydratase
  • Fatty acids
  • Periplasmic expression
  • Whole cell biotransformation

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