Abstract
We report an innovative fast antimicrobial enzymatic assay with high sensitivity by nanopore plasmonic membrane-based integrated optofluidic system where whole processes of enzymatic assay including bacteria enrichment, lysis, and detection can be performed on a single platform within 30 minutes. An effective photothermal lysis of pathogens was successfully accomplished via pulsed light modulations on plasmonic nanopore membrane, which allows localized heating in the vicinity of bacteria on the membrane and extraction of target enzyme without any lose their activities.
| Original language | English |
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| Title of host publication | 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 1860-1862 |
| Number of pages | 3 |
| ISBN (Electronic) | 9781510897571 |
| State | Published - 2018 |
| Event | 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 - Kaohsiung, Taiwan, Province of China Duration: 11 Nov 2018 → 15 Nov 2018 |
Publication series
| Name | 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 |
|---|---|
| Volume | 3 |
Conference
| Conference | 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 |
|---|---|
| Country/Territory | Taiwan, Province of China |
| City | Kaohsiung |
| Period | 11/11/18 → 15/11/18 |
Bibliographical note
Funding Information:The authors acknowledge the financial support provided by the National Institutes of Health (NIH) (R01 AI117064-01).
Publisher Copyright:
Copyright © (2018) by Chemical and Biological Microsystems Society. All rights reserved.
Keywords
- Antimicrobial resistance
- Bacterial enrichment
- Urinary tract infections (UTIs)
- β-lactamase